Invitrogen

Synapsin 1 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

22 References

View all (32) Synapsin 1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Synapsin 1 Antibody in Western Blot (WB)

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Synapsin 1 Antibody (51-5200) in WB

Western blot was performed using anti-Synapsin1 Polyclonal Antibody (Product # 51-5200) and 78 kDa band corresponding to Synapsin1 was observed across cell lines tested and tissues except Mouse Lung and Heart which are reported to be negative. Whole cell extracts of (30 µg lysate) of IMR-32 (Lane 1), PC-12 (Lane 2), tissue extracts of Mouse Brain (Lane 3), Mouse Lung (Lane 4) and Mouse Heart (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocell... View More

Synapsin 1 Antibody in Immunocytochemistry (ICC/IF)

Synapsin 1 Antibody in Immunohistochemistry (IHC)

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Product Details

51-5200

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

1:2,000

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000-1:2,000

View 6 publications 6 publications

Immunoprecipitation (IP)

1 µg/200 µg rat brain lysate

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified bovine brain Synapsin 1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Antigen affinity chromatography

Storage buffer

whole serum diluted in 5mM Ammonium bicarbonate

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533909

Product Specific Information

The antibody should be reconstituted in 50 uL phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody should be aliquot and stored at -20C.

Target Information

SYN1 (synapsin 1) is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins that associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains and are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. Synapsin 1 plays a role in regulation of axonogenesis and synaptogenesis. It serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Adnp-mutant mice with cognitive inflexibility, CaMKIIα hyperactivity, and synaptic plasticity deficits.

Molecular psychiatry

Cho H,Yoo T,Moon H,Kang H,Yang Y,Kang M,Yang E,Lee D,Hwang D,Kim H,Kim D,Kim JY,Kim E

51-5200 was used in Western Blotting to report synaptic plasticity deficits associated with cognitive inflexibility and CaMKIIα hyperactivity in Adnp-HT mice.

Tue Aug 01 00:00:00 EDT 2023

Analysis of the distribution of vagal afferent projections from different peripheral organs to the nucleus of the solitary tract in rats.

The Journal of comparative neurology

Bassi JK,Connelly AA,Butler AG,Liu Y,Ghanbari A,Farmer DGS,Jenkins MW,Melo MR,McDougall SJ,Allen AM

51-5200 was used in Western Blotting to conclude that specific viscerosensory information is distributed widely within the NTS and that the coding of this input is probably determined by the intrinsic properties and projections of the second-order neuron.

Thu Dec 01 00:00:00 EST 2022

Analysis of the distribution of vagal afferent projections from different peripheral organs to the nucleus of the solitary tract in rats.

The Journal of comparative neurology

Bassi JK,Connelly AA,Butler AG,Liu Y,Ghanbari A,Farmer DGS,Jenkins MW,Melo MR,McDougall SJ,Allen AM

Thu Dec 01 00:00:00 EST 2022

Neurobiology of aging

Evans AK,Park HH,Saw NL,Singhal K,Ogawa G,Leib RD,Shamloo M

51-5200 was used in Western Blotting to examine effects of age and acute beta-blocker administration on LC and hippocampus pathology, neuroinflammation and learning and memory behavior in mice.

Fri Oct 01 00:00:00 EDT 2021

Intranasal Dantrolene as a Disease-Modifying Drug in Alzheimer 5XFAD Mice.

Journal of Alzheimer's disease : JAD

Shi Y,Zhang L,Gao X,Zhang J,Ben Abou M,Liang G,Meng Q,Hepner A,Eckenhoff MF,Wei H

51-5200 was used in Western Blotting to compare the effectiveness and safety of intranasal versus subcutaneous administration of dantrolene in 5XFAD Alzheimer's disease (AD) mice.

Thu Jun 03 00:00:00 EDT 2021

Effects of Dexamethasone on Remodeling of the Hippocampal Synaptic Filamentous Actin Cytoskeleton in a Model of Pilocarpine-induced Status Epilepticus.

International journal of medical sciences

Yang N,Zhang Y,Wang JT,Chen C,Song Y,Liang JM,Ma DH,Zhang YF

51-5200 was used in Immunohistochemistry-immunofluorescence to indicate that treatment with dexamethasone stabilizes the synaptic F-actin cytoskeleton and reduces the damage to the brain due to status epilepticus (SE), which is expected to be beneficial in alleviating delayed neuron damage and the process of epileptogenesis.

Tue Jun 01 00:00:00 EDT 2021

Effects of Dexamethasone on Remodeling of the Hippocampal Synaptic Filamentous Actin Cytoskeleton in a Model of Pilocarpine-induced Status Epilepticus.

International journal of medical sciences

Yang N,Zhang Y,Wang JT,Chen C,Song Y,Liang JM,Ma DH,Zhang YF

Tue Jun 01 00:00:00 EDT 2021

Transplantation of iPS cell-derived neural progenitors overexpressing SDF-1α increases regeneration and functional recovery after ischemic stroke.

Oncotarget

Chau M,Deveau TC,Song M,Wei ZZ,Gu X,Yu SP,Wei L

51-5200 was used in Immunocytochemistry to show SDF-1α upregulation in transplanted cells may be a therapeutic strategy to enhance endogenous neurovascular repair after ischemic stroke in adult mice.

Tue Nov 14 00:00:00 EST 2017

Biophysical constraints of optogenetic inhibition at presynaptic terminals.

Nature neuroscience

Mahn M,Prigge M,Ron S,Levy R,Yizhar O

51-5200 was used in immunocytochemistry to study presynaptic terminals and biophyscial constraints of optogeneitc inhibition

Fri Apr 01 00:00:00 EDT 2016

Biophysical constraints of optogenetic inhibition at presynaptic terminals.

Nature neuroscience

Mahn M,Prigge M,Ron S,Levy R,Yizhar O

51-5200 was used in immunocytochemistry to study presynaptic terminals and biophyscial constraints of optogeneitc inhibition

Fri Apr 01 00:00:00 EDT 2016

Glimepiride protects neurons against amyloid-β-induced synapse damage.

Neuropharmacology

Osborne C,West E,Nolan W,McHale-Owen H,Williams A,Bate C

51-5200 was used in western blot to investigate how glimepiride protects neurons against synapse damage induced by Aβ.

Mon Feb 01 00:00:00 EST 2016

The progressive changes of filamentous actin cytoskeleton in the hippocampal neurons after pilocarpine-induced status epilepticus.

Epilepsy research

Xiong T,Liu J,Dai G,Hou Y,Tan B,Zhang Y,Li S,Song Y,Liu H,Li Y,Li Y

51-5200 was used in immunohistochemistry to assess the temporal and spatial changes of F-actin during the period of epileptogenesis

Tue Dec 01 00:00:00 EST 2015

cAMP-Inhibits Cytoplasmic Phospholipase A₂ and Protects Neurons against Amyloid-β-Induced Synapse Damage.

Biology

Bate C,Williams A

51-5200 was used in western blot to study Aβ-induced activation of cPLA.

Wed Sep 16 00:00:00 EDT 2015

iPSC Transplantation increases regeneration and functional recovery after ischemic stroke in neonatal rats.

Stem cells (Dayton, Ohio)

Chau MJ,Deveau TC,Song M,Gu X,Chen D,Wei L

51-5200 was used in immunocytochemistry to test if transplanted iPSC-derived neural progenitor cells increase regeneration after stroke in neonatal rats.

Mon Dec 01 00:00:00 EST 2014

CCL2-ethanol interactions and hippocampal synaptic protein expression in a transgenic mouse model.

Frontiers in integrative neuroscience

Gruol DL,Vo K,Bray JG,Roberts AJ

51-5200 was used in western blot to study the effect of ethanol exposure on CCL2 secretion and hippocampal synaptic protein expression

Mon Apr 28 00:00:00 EDT 2014

The rearrangement of filamentous actin in mossy fiber synapses in pentylenetetrazol-kindled C57BL/6 mice.

Epilepsy research

Zhang YF,Li SL,Xiong TQ,Yang LB,Li YN,Tan BH,Liu Q,Li YC

Wed Jan 01 00:00:00 EST 2014

Increased astrocyte expression of IL-6 or CCL2 in transgenic mice alters levels of hippocampal and cerebellar proteins.

Frontiers in cellular neuroscience

Gruol DL,Vo K,Bray JG

51-5200 was used in western blot to study IL-6 and CCL2 in the central nervous system

Mon Sep 01 00:00:00 EDT 2014

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bertran-Gonzalez J,Laurent V,Chieng BC,Christie MJ,Balleine BW

Published figure using Synapsin 1 polyclonal antibody (Product # 51-5200) in Immunofluorescence

Wed Oct 09 00:00:00 EDT 2013

Altered synaptic transmission in the hippocampus of transgenic mice with enhanced central nervous systems expression of interleukin-6.

Brain, behavior, and immunity

Nelson TE,Olde Engberink A,Hernandez R,Puro A,Huitron-Resendiz S,Hao C,De Graan PN,Gruol DL

51-5200 was used in immunohistochemistry - frozen section to assess the role of IL-6 in neuronal function.

Wed Aug 01 00:00:00 EDT 2012

Neurodegeneration induced by clustering of sialylated glycosylphosphatidylinositols of prion proteins.

The Journal of biological chemistry

Bate C,Williams A

51-5200 was used in western blot to study the impact of glycosylphosphatidylinositol anchoring on prion disease.

Fri Mar 09 00:00:00 EST 2012

Neurodegeneration induced by clustering of sialylated glycosylphosphatidylinositols of prion proteins.

The Journal of biological chemistry

Bate C,Williams A

51-5200 was used in western blot to study the impact of glycosylphosphatidylinositol anchoring on prion disease.

Fri Mar 09 00:00:00 EST 2012

Altered hippocampal synaptic transmission in transgenic mice with astrocyte-targeted enhanced CCL2 expression.

Brain, behavior, and immunity

Nelson TE,Hao C,Manos J,Ransohoff RM,Gruol DL

Wed Jun 01 00:00:00 EDT 2011

Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6.

Journal of neuroimmunology

Gruol DL,Puro A,Hao C,Blakely P,Janneke E,Vo K

Fri Oct 28 00:00:00 EDT 2011

Spine formation and maturation in the developing rat auditory cortex.

The Journal of comparative neurology

Schachtele SJ,Losh J,Dailey ME,Green SH

Published figure using Synapsin 1 polyclonal antibody (Product # 51-5200) in Immunofluorescence

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Brain protein 4.1; synapsin 1; Synapsin I; Synapsin-1; truncated synapsin 1-S

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Gene Aliases: Syn-1; SYN1; Syn1-S; SYN1a; SYN1b; SYNI

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UniProt ID: (Bovine) P17599, (Human) P17600, (Rat) P09951, (Mouse) O88935

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Entrez Gene ID: (Bovine) 281510, (Human) 6853, (Rat) 24949, (Mouse) 20964

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Function(s)

actin binding ATP binding catalytic activity transporter activity protein binding protein kinase binding calcium-dependent protein binding protein dimerization activity membrane trafficking regulatory protein

Process(es)

chemical synaptic transmission neurotransmitter secretion regulation of neurotransmitter secretion regulation of synaptic vesicle exocytosis metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Synapsin 1 Polyclonal Antibody

Synapsin 1 Antibody (51-5200) in WB

Product Details

51-5200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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