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Invitrogen
CD124 Recombinant Rabbit Monoclonal Antibody (Astra-1), Alexa Fluor™ 488, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CD124 Antibody (53-1241-82) in Flow
BALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82) and 0.03 µg of Recombinant Rabbit IgG Monoclonal Isotype Control, Alexa Fluor 488 (Product # 53-4616-82) (left) or 0.03 µg of CD124 Recombinant Rabbit Monoclonal Antibody, Alexa Fluor 488 (right). Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
53-1241-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
Astra-1
Immunogen
Protein corresponding to mouse CD124 (aa26-aa233)
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer/PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2848421
Product Specific Information
Description: This Astra-1 recombinant rabbit monoclonal antibody reacts with mouse CD124, which is also known as the IL4 receptor alpha (IL4RA) chain.
Applications Tested: This Astra-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor alpha chain; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Interleukin-4 receptor subunit alpha
Gene Aliases: CD124; Il4r; Il4ra
UniProt ID: (Mouse) P16382
Entrez Gene ID: (Mouse) 16190
immune system process
production of molecular mediator involved in inflammatory response
positive regulation of immunoglobulin production
immunoglobulin mediated immune response
regulation of cell proliferation
defense response to protozoan
positive regulation of macrophage activation
positive regulation of mast cell degranulation
negative regulation of T-helper 1 cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of chemokine secretion
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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