Invitrogen

Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

15 Published Figures

27 References

View all (9) Myosin 4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Myosin 4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

Myosin 4 Antibody (53-6503-82) in ICC/IF

Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 and C2C12 differentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor 488, eBioscience™ (Product # 53-6503-82) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Dia... View More

Myosin 4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Myosin 4 Antibody in Immunohistochemistry (IHC)

Myosin 4 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

53-6503-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 13 publications 13 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Chimpanzee, Cat, Guinea pig, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MF20

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671272

Product Specific Information

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.

The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.

Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Uterus-Inspired Niche Drives Blastocyst Development to the Early Organogenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Gu Z,Guo J,Zhai J,Feng G,Wang X,Gao Z,Li K,Ji S,Wang L,Xu Y,Chen X,Wang Y,Guo S,Yang M,Li L,Han H,Jiang L,Wen Y,Wang L,Hao J,Li W,Wang S,Wang H,Gu Q

53-6503-82 was used in Immunocytochemistry-immunofluorescence to establish a uterus-inspired niche (UN) by grafting collagen gels onto polydimethylsiloxane, systematically varying concentrations and thicknesses of collagen gel to mimic the mechanical properties and microstructures of the mouse uterus, resulting in a robust system that supports embryo invasion and promotes early organogenesis, offering a valuable tool for developmental biology and tissue engineering.

Sat Oct 01 00:00:00 EDT 2022

TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice.

Cell death & disease

Al-Zaeed N,Budai Z,Szondy Z,Sarang Z

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Sat Jun 12 00:00:00 EDT 2021

TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice.

Cell death & disease

Al-Zaeed N,Budai Z,Szondy Z,Sarang Z

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Sat Jun 12 00:00:00 EDT 2021

Impaired Skeletal Muscle Development and Regeneration in Transglutaminase 2 Knockout Mice.

Cells

Budai Z,Al-Zaeed N,Szentesi P,Halász H,Csernoch L,Szondy Z,Sarang Z

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Tue Nov 09 00:00:00 EST 2021

Impaired Skeletal Muscle Development and Regeneration in Transglutaminase 2 Knockout Mice.

Cells

Budai Z,Al-Zaeed N,Szentesi P,Halász H,Csernoch L,Szondy Z,Sarang Z

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Tue Nov 09 00:00:00 EST 2021

In vivo detection of programmed cell death during mouse heart development.

Cell death and differentiation

Martínez-Lagunas K,Yamaguchi Y,Becker C,Geisen C,DeRuiter MC,Miura M,Fleischmann BK,Hesse M

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

Comprehensive histological investigation of age-related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion.

International journal of cosmetic science

Gomi T,Imamura T

53-6503 was used in Immunohistochemistry-immunofluorescence to collect and study histological and molecular data on age-dependent alterations in the upper lip vermillion.

Sat Aug 01 00:00:00 EDT 2020

TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice.

Scientific reports

Ono Y,Maejima Y,Saito M,Sakamoto K,Horita S,Shimomura K,Inoue S,Kotani J

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunofluorescence

Mon Jan 20 00:00:00 EST 2020

TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice.

Scientific reports

Ono Y,Maejima Y,Saito M,Sakamoto K,Horita S,Shimomura K,Inoue S,Kotani J

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunofluorescence

Mon Jan 20 00:00:00 EST 2020

Data on the proliferation and differentiation of C2C12 myoblast treated with branched-chain ketoacid dehydrogenase kinase inhibitor.

Data in brief

Sato Y,Tate H,Yoshizawa F,Sato Y

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2020

hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum Neurotoxins.

Frontiers in pharmacology

Lamotte JD,Roqueviere S,Gautier H,Raban E,Bouré C,Fonfria E,Krupp J,Nicoleau C

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunocytochemistry

Tue Feb 02 00:00:00 EST 2021

Assessment of different strategies for scalable production and proliferation of human myoblasts.

Cell proliferation

Chua MJ,Yildirim ED,Tan JE,Chua YB,Low SC,Ding SLS,Li CW,Jiang Z,Teh BT,Yu K,Shyh-Chang N

53-6503 was used in Immunocytochemistry-immunoflourescence to show that, while all the strategies above are suboptimal at generating bona fide human myoblasts that can both proliferate and differentiate robustly, chemically defined hESC-monolayer-myoblasts show the most promise in differentiation potential.

Wed May 01 00:00:00 EDT 2019

Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell-derived skeletal myocytes.

Biotechnology and bioengineering

Jiwlawat N,Lynch EM,Napiwocki BN,Stempien A,Ashton RS,Kamp TJ,Crone WC,Suzuki M

53-6503-82 was used in Immunohistochemistry to demonstrate that human induced pluripotent stem cell (iPSC)-derived myogenic progenitors formed highly-aligned myotubes and contracted when seeded on two-dimensional micropatterned platforms.

Sun Sep 01 00:00:00 EDT 2019

Peptidyl-prolyl cis-trans isomerase NIMA interacting 1 regulates skeletal muscle fusion through structural modification of Smad3 in the linker region.

Journal of cellular physiology

Islam R,Yoon H,Shin HR,Bae HS,Kim BS,Yoon WJ,Woo KM,Baek JH,Lee YS,Ryoo HM

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunocytochemistry

Sat Dec 01 00:00:00 EST 2018

Classification of C2C12 cells at differentiation by convolutional neural network of deep learning using phase contrast images.

Human cell

Niioka H,Asatani S,Yoshimura A,Ohigashi H,Tagawa S,Miyake J

53-6503 was used in Immunocytochemistry to develop the convolutional neural network algorithm for automatic cellular differentiation recognition of the myogenic C12C12 cell line.

Mon Jan 01 00:00:00 EST 2018

Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis.

Nanotheranostics

Shin YC,Kim C,Song SJ,Jun S,Kim CS,Hong SW,Hyon SH,Han DW,Oh JW

53-6503 was used in Immunocytochemistry-immunoflourescence to fabricate poly(lactic-co-glycolic acid) nanofibre sheets decorated with graphene oxide and RGD peptid, as biofunctional scaffolds.

Wed Nov 20 00:00:00 EST 2019

NOTCH1-Dependent Nitric Oxide Signaling Deficiency in Hypoplastic Left Heart Syndrome Revealed Through Patient-Specific Phenotypes Detected in Bioengineered Cardiogenesis.

Stem cells (Dayton, Ohio)

Hrstka SC,Li X,Nelson TJ

53-6503 was used in Immunocytochemistry-immunoflourescence to study a mechanistic basis through which NOTCH1 contributes to heart development.

Sat Apr 01 00:00:00 EDT 2017

An Engineered Optogenetic Switch for Spatiotemporal Control of Gene Expression, Cell Differentiation, and Tissue Morphogenesis.

ACS synthetic biology

Polstein LR,Juhas M,Hanna G,Bursac N,Gersbach CA

53-6503 was used in Immunocytochemistry-immunofluorescence to apply optogenetics to control cell differentiation and new tissue formation.

Fri Nov 17 00:00:00 EST 2017

Targeted Ablation of Periostin-Expressing Activated Fibroblasts Prevents Adverse Cardiac Remodeling in Mice.

Circulation research

Kaur H,Takefuji M,Ngai CY,Carvalho J,Bayer J,Wietelmann A,Poetsch A,Hoelper S,Conway SJ,Möllmann H,Looso M,Troidl C,Offermanns S,Wettschureck N

53-6503 was used in Immunohistochemistry to study the effect of activated cardiac fibroblast ablation on cardiac remodeling.

Fri Jun 10 00:00:00 EDT 2016

Natural underlying mtDNA heteroplasmy as a potential source of intra-person hiPSC variability.

The EMBO journal

Perales-Clemente E,Cook AN,Evans JM,Roellinger S,Secreto F,Emmanuele V,Oglesbee D,Mootha VK,Hirano M,Schon EA,Terzic A,Nelson TJ

53-6503 was used in Flow cytometry/Cell sorting to characterise the mitochondrial DNA of human induced pluripotent stem cells for use as a selection criteria.

Thu Sep 15 00:00:00 EDT 2016

Ultrastructure of medial rectus muscles in patients with intermittent exotropia.

Eye (London, England)

Yao J,Wang X,Ren H,Liu G,Lu P

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Western Blot

Fri Jan 01 00:00:00 EST 2016

Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment.

Integrative biology : quantitative biosciences from nano to macro

Gibson TM,Gersbach CA

53-6503 was used in Immunofluorescence to characterise the differentiation of single skeletal muscle cells.

Mon Jun 01 00:00:00 EDT 2015

Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment.

Integrative biology : quantitative biosciences from nano to macro

Gibson TM,Gersbach CA

53-6503 was used in Immunofluorescence to characterise the differentiation of single skeletal muscle cells.

Mon Jun 01 00:00:00 EDT 2015

iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.

Circulation. Cardiovascular genetics

Martinez-Fernandez A,Nelson TJ,Reyes S,Alekseev AE,Secreto F,Perez-Terzic C,Beraldi R,Sung HK,Nagy A,Terzic A

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.

Circulation. Cardiovascular genetics

Martinez-Fernandez A,Nelson TJ,Reyes S,Alekseev AE,Secreto F,Perez-Terzic C,Beraldi R,Sung HK,Nagy A,Terzic A

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.

Circulation. Cardiovascular genetics

Martinez-Fernandez A,Nelson TJ,Reyes S,Alekseev AE,Secreto F,Perez-Terzic C,Beraldi R,Sung HK,Nagy A,Terzic A

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Inhibition of TGFβ signaling increases direct conversion of fibroblasts to induced cardiomyocytes.

PloS one

Ifkovits JL,Addis RC,Epstein JA,Gearhart JD

Published figure using Myosin 4 monoclonal antibody (Product # 53-6503-82) in Immunocytochemistry

Tue Jan 20 00:00:00 EST 2015

Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success.

Journal of molecular and cellular cardiology

Addis RC,Ifkovits JL,Pinto F,Kellam LD,Esteso P,Rentschler S,Christoforou N,Epstein JA,Gearhart JD

53-6503 was used in Immunohistochemistry to conclude that HNGMT produces iCMs more efficiently than previously published methods.

Mon Jul 01 00:00:00 EDT 2013

Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success.

Journal of molecular and cellular cardiology

Addis RC,Ifkovits JL,Pinto F,Kellam LD,Esteso P,Rentschler S,Christoforou N,Epstein JA,Gearhart JD

53-6503 was used in Immunohistochemistry to conclude that HNGMT produces iCMs more efficiently than previously published methods.

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product

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Gene Aliases: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf

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UniProt ID: (Human) Q9Y623, (Dog) Q076A5, (Rabbit) Q28641, (Mouse) Q5SX39, (Rat) Q29RW1

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Entrez Gene ID: (Human) 4622, (Bovine) 408020, (Dog) 479502, (Rabbit) 100009548, (Rabbit) 100125991, (Guinea pig) 100726456, (Mouse) 17884, (Rat) 360543

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Function(s)

microfilament motor activity double-stranded RNA binding actin binding calmodulin binding ATP binding ATPase activity motor activity actin filament binding nucleotide binding molecular_function

Process(es)

muscle contraction response to activity actin filament-based movement muscle filament sliding ATP metabolic process metabolic process response to gravity response to muscle activity response to muscle stretch muscle structure development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™

Myosin 4 Antibody (53-6503-82) in ICC/IF

Product Details

53-6503-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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