Invitrogen

CD284 (TLR4) Monoclonal Antibody (UT41), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

21 References

View all (67) TLR4 (CD284) antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD284 (TLR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 15

CD284 (TLR4) Antibody (53-9041-82) in Flow

C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells were stained with 0.5 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or 0.5 µg of CD284 (TLR4) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD284 (TLR4) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD284 (TLR4) Antibody in Immunohistochemistry (IHC)

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Product Details

53-9041-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UT41

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469944

Product Specific Information

Description: The UT41 monoclonal antibody was generated against BaF3 cells overexpressing mouse TLR4. So far, at least twelve members of the Toll family have been identified. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: This UT41 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UT41 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Macrophage polarization is involved in liver fibrosis induced by β ₁-adrenoceptor autoantibody.

Acta biochimica et biophysica Sinica

Wu Y,Fan X,Yu H,Liu J,Duan Y,Zhang S,Yan L,Du Y,Liu H

53-9041-82 was used in Flow cytometry/Cell sorting to report the effect of macrophage disorder upon liver fibrosis and dysfunctions caused by β1-AA, and propose a novel mechanism responsible for the effect of β1-AA on hepatic function.

Thu Aug 25 00:00:00 EDT 2022

Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages.

PloS one

Kitaura A,Nishinaka T,Hamasaki S,Hatipoglu OF,Wake H,Nishibori M,Mori S,Nakao S,Takahashi H

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Thu Jun 24 00:00:00 EDT 2021

Erlotinib protests against LPS-induced parthanatos through inhibiting macrophage surface TLR4 expression.

Cell death discovery

Xue Q,Liu X,Chen C,Zhang X,Xie P,Liu Y,Zhou S,Tang J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells.

PLoS pathogens

Akhtar MN,Mishra M,Yadav V,Yadav M,Gujar R,Lal S,Kumar R,Khatri N,Sen P

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Overexpression of Galectin 3 in Pancreatic β Cells Amplifies β-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice.

Frontiers in endocrinology

Petrovic I,Pejnovic N,Ljujic B,Pavlovic S,Miletic Kovacevic M,Jeftic I,Djukic A,Draginic N,Andjic M,Arsenijevic N,Lukic ML,Jovicic N

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Immunohistochemistry

Wed Feb 03 00:00:00 EST 2021

Sphingomyelin Synthase 2 Inhibition Ameliorates Cerebral Ischemic Reperfusion Injury Through Reducing the Recruitment of Toll-Like Receptor 4 to Lipid Rafts.

Journal of the American Heart Association

Xue J,Yu Y,Zhang X,Zhang C,Zhao Y,Liu B,Zhang L,Wang L,Chen R,Gao X,Jiao P,Song G,Jiang XC,Qin S

53-9041 was used in Flow cytometry/Cell sorting to investigate the role of SMS2 in a mouse model of cerebral I/R.

Tue Nov 19 00:00:00 EST 2019

Provocative decompression causes diffuse vascular injury in mice mediated by microparticles containing interleukin-1β.

Journal of applied physiology (Bethesda, Md. : 1985)

Thom SR,Bhopale VM,Yu K,Yang M

53-9041-82 was used in Flow cytometry/Cell sorting to demonstrate that elevations of circulating MPs containing up to 9-fold higher concentrations of IL-1β occur while mice are exposed to high air pressure whereas smaller particles carrying proteins specific to exosomes are not elevated.

Mon Oct 01 00:00:00 EDT 2018

Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.

Cell host & microbe

Thevaranjan N,Puchta A,Schulz C,Naidoo A,Szamosi JC,Verschoor CP,Loukov D,Schenck LP,Jury J,Foley KP,Schertzer JD,Larché MJ,Davidson DJ,Verdú EF,Surette MG,Bowdish DME

53-9041 was used in Flow cytometry/Cell sorting to study the potential of changes to the microbiota as a strategy for reducing age-associated inflammation and the accompanying morbidity.

Wed Apr 12 00:00:00 EDT 2017

Tissue damage negatively regulates LPS-induced macrophage necroptosis.

Cell death and differentiation

Li Z,Scott MJ,Fan EK,Li Y,Liu J,Xiao G,Li S,Billiar TR,Wilson MA,Jiang Y,Fan J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Differential effects of gram-positive and gram-negative bacterial products on morphine induced inhibition of phagocytosis.

Scientific reports

Ninkovic J,Anand V,Dutta R,Zhang L,Saluja A,Meng J,Koodie L,Banerjee S,Roy S

Fri Feb 19 00:00:00 EST 2016

Differential effects of gram-positive and gram-negative bacterial products on morphine induced inhibition of phagocytosis.

Scientific reports

Ninkovic J,Anand V,Dutta R,Zhang L,Saluja A,Meng J,Koodie L,Banerjee S,Roy S

Fri Feb 19 00:00:00 EST 2016

Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production.

Proceedings of the National Academy of Sciences of the United States of America

Millrine D,Tei M,Gemechu Y,Kishimoto T

53-9041-82 was used in Immunocytochemistry to propose that release of Rabex-5 from complex with Cereblon enables the suppression of immune responses, contributing to the anti-inflammatory properties of immunomodulatory drugs (IMiDs).

Tue Sep 20 00:00:00 EDT 2016

The binding capability of plasma phospholipid transfer protein, but not HDL pool size, is critical to repress LPS induced inflammation.

Scientific reports

Yu Y,Cui Y,Zhao Y,Liu S,Song G,Jiao P,Li B,Luo T,Guo S,Zhang X,Wang H,Jiang XC,Qin S

53-9041 was used in Flow cytometry/Cell sorting to determine the exact role of phospholipid transfer protein in lipopolysaccharide-induced inflammation.

Tue Feb 09 00:00:00 EST 2016

Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model.

Journal of neuroinflammation

Dutta R,Roy S

53-9041 was used in Flow cytometry/Cell sorting to explore the role of systemic infection with Streptococcus pneumoniae in the recruitment of peripheral leukocytes into the central nervous system.

Sat Jun 20 00:00:00 EDT 2015

Carbon monoxide-based therapy ameliorates acute pancreatitis via TLR4 inhibition.

The Journal of clinical investigation

Xue J,Habtezion A

53-9041 was used in Flow cytometry/Cell sorting to investigate a therapeutic role for CO and CO-primed cells in acute pancreatitis.

Wed Jan 01 00:00:00 EST 2014

A critical role of toll-like receptor 4 (TLR4) and its' in vivo ligands in basal radio-resistance.

Cell death & disease

Liu C,Zhang C,Mitchel RE,Cui J,Lin J,Yang Y,Liu X,Cai J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Thu May 30 00:00:00 EDT 2013

Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A.

PloS one

Casella CR,Mitchell TC

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Rip2 deficiency leads to increased atherosclerosis despite decreased inflammation.

Circulation research

Levin MC,Jirholt P,Wramstedt A,Johansson ME,Lundberg AM,Trajkovska MG,Ståhlman M,Fogelstrand P,Brisslert M,Fogelstrand L,Yan ZQ,Hansson GK,Björkbacka H,Olofsson SO,Borén J

53-9041 was used in Flow cytometry/Cell sorting to show that lipid accumulation and inflammation are dissociated in the vessel wall in mice with Rip2/ macrophages.

Fri Nov 11 00:00:00 EST 2011

Yersinia pestis and host macrophages: immunodeficiency of mouse macrophages induced by YscW.

Immunology

Bi Y,Du Z,Han Y,Guo Z,Tan Y,Zhu Z,Yang R

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

Candesartan inhibits Toll-like receptor expression and activity both in vitro and in vivo.

Atherosclerosis

Dasu MR,Riosvelasco AC,Jialal I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Pioglitazone inhibits Toll-like receptor expression and activity in human monocytes and db/db mice.

Endocrinology

Dasu MR,Park S,Devaraj S,Jialal I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9041-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD284; lipopolysaccharide response; Toll-like receptor 4

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Gene Aliases: Lps; Ly87; Ran/M1; Rasl2-8; Tlr4

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UniProt ID: (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 21898

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Function(s)

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding phosphatidylinositol 3-kinase binding

Process(es)

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD284 (TLR4) Monoclonal Antibody (UT41), Alexa Fluor™ 488, eBioscience™

CD284 (TLR4) Antibody (53-9041-82) in Flow

Product Details

53-9041-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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