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Invitrogen
KLRG1 Monoclonal Antibody (13F12F2), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 3
KLRG1 Antibody (53-9488-42) in Flow
LEFT: Staining of normal human peripheral blood cells with Anti-Human CD28 eFluor® 450 (Product # 48-0289-42) and Anti-Human KLRG1 Alexa Fluor® 488. CD8+ cells in the lymphocyte gate were used for analysis. RIGHT: Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE-eFluor® 610 (Product # 61-0567-42) and Anti-Human KLRG1 Alexa Fluor® 488. Cells in the lymphocyte gate were used for analysis.
Product Details
53-9488-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
13F12F2
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574451
Product Specific Information
Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain. This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells. KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function. Cadherin-E, -N, and -R are ligands for KLRG1. Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience.
The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies.
Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; Killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; MAFA-like receptor; Mast cell function-associated antigen; mast cell function-associated antigen (ITIM-containing); natural killer cell receptor
Gene Aliases: 2F1; CLEC15A; KLRG1; MAFA; MAFA-2F1; MAFA-L; MAFA-LIKE; MAFAL
UniProt ID: (Human) Q96E93
Entrez Gene ID: (Human) 10219
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