Invitrogen

SOX2 Monoclonal Antibody (Btjce), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

60 Published Figures

48 References

View all (90) SOX2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOX2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 65

SOX2 Antibody (53-9811-82) in ICC/IF

Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # 53-9811-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin ... View More

SOX2 Antibody in Immunohistochemistry (IHC)

SOX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

SOX2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

SOX2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

SOX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Expression of SOX2 was observed in NTERA-2 as opposed to HeLa cell line using SOX2 Monoclonal Antibody (Product # 53-9811-80) in ICC. {RE}

Product Details

53-9811-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 22 publications 22 publications

Flow Cytometry (Flow)

0.25 µg/mL

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Btjce

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574479

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized Ntera cells at less than or equal to 10 µg/mL. In flow cytometry it can be used at 0.25 µg/mL using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Bacterial expression of region after aa135 (homology between species but not to other family members).

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Thioredoxin facilitates hepatocellular carcinoma stemness and metastasis by increasing BACH1 stability to activate the AKT/mTOR pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang C,Zhang L,Cao M,Fu Z,Wang H,Zhang S,Zhu K,Hou Z,Cui J,Yue P,Guo H,Zhang T

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Western Blot

Thu Jun 01 00:00:00 EDT 2023

A gastruloid model of the interaction between embryonic and extra-embryonic cell types.

Journal of tissue engineering

Bérenger-Currias NM,Mircea M,Adegeest E,van den Berg PR,Feliksik M,Hochane M,Idema T,Tans SJ,Semrau S

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Sat Jul 16 00:00:00 EDT 2022

A gastruloid model of the interaction between embryonic and extra-embryonic cell types.

Journal of tissue engineering

Bérenger-Currias NM,Mircea M,Adegeest E,van den Berg PR,Feliksik M,Hochane M,Idema T,Tans SJ,Semrau S

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Sat Jul 16 00:00:00 EDT 2022

Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.

Experimental and therapeutic medicine

Hiramatsu N,Yamamoto N,Kato Y,Nagai N,Isogai S,Imaizumi K

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Mon Aug 01 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential.

Developmental cell

Frederico B,Martins I,Chapela D,Gasparrini F,Chakravarty P,Ackels T,Piot C,Almeida B,Carvalho J,Ciccarelli A,Peddie CJ,Rogers N,Briscoe J,Guillemot F,Schaefer AT,Saúde L,Reis e Sousa C

53-9811-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to report that DNGR-1 lineage tracing in mice identifies an ependymal cell subset, wherein resides latent regenerative potential.

Mon Aug 22 00:00:00 EDT 2022

DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential.

Developmental cell

Frederico B,Martins I,Chapela D,Gasparrini F,Chakravarty P,Ackels T,Piot C,Almeida B,Carvalho J,Ciccarelli A,Peddie CJ,Rogers N,Briscoe J,Guillemot F,Schaefer AT,Saúde L,Reis e Sousa C

Mon Aug 22 00:00:00 EDT 2022

Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model.

eLife

Hoye ML,Calviello L,Poff AJ,Ejimogu NE,Newman CR,Montgomery MD,Ou J,Floor SN,Silver DL

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Tue Jun 28 00:00:00 EDT 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Three-Dimensional Vessels-on-a-Chip Based on hiPSC-derived Vascular Endothelial and Smooth Muscle Cells.

Current protocols

Bulut M,Vila Cuenca M,de Graaf M,van den Hil FE,Mummery CL,Orlova VV

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2022

Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies.

PloS one

Choy Buentello D,Koch LS,Trujillo-de Santiago G,Alvarez MM,Broersen K

53-9811-82 was used in Immunohistochemistry to show that embryoid bodies (EBs) generated by the protocols introduced here successfully developed into neural organoids and expressed the relevant markers associated with their lineages.

Thu May 12 00:00:00 EDT 2022

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.

Acta neuropathologica communications

Lambert E,Saha O,Soares Landeira B,Melo de Farias AR,Hermant X,Carrier A,Pelletier A,Gadaut J,Davoine L,Dupont C,Amouyel P,Bonnefond A,Lafont F,Abdelfettah F,Verstreken P,Chapuis J,Barois N,Delahaye F,Dermaut B,Lambert JC,Costa MR,Dourlen P

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Sat Jan 08 00:00:00 EST 2022

Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.

Cell stem cell

Kinoshita M,Barber M,Mansfield W,Cui Y,Spindlow D,Stirparo GG,Dietmann S,Nichols J,Smith A

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Thu Mar 04 00:00:00 EST 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.

Journal of neuroscience research

Ahn LY,Coatti GC,Liu J,Gumus E,Schaffer AE,Miranda HC

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Fri Jan 01 00:00:00 EST 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Fri Nov 05 00:00:00 EDT 2021

Induction of inverted morphology in brain organoids by vertical-mixing bioreactors.

Communications biology

Suong DNA,Imamura K,Inoue I,Kabai R,Sakamoto S,Okumura T,Kato Y,Kondo T,Yada Y,Klein WL,Watanabe A,Inoue H

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

Modulating mesendoderm competence during human germ layer differentiation.

Cell reports

Valcourt JR,Huang RE,Kundu S,Venkatasubramanian D,Kingston RE,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Tue Nov 09 00:00:00 EST 2021

Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.

Nature communications

Kojic M,Gawda T,Gaik M,Begg A,Salerno-Kochan A,Kurniawan ND,Jones A,Drożdżyk K,Kościelniak A,Chramiec-Głąbik A,Hediyeh-Zadeh S,Kasherman M,Shim WJ,Sinniah E,Genovesi LA,Abrahamsen RK,Fenger CD,Madsen CG,Cohen JS,Fatemi A,Stark Z,Lunke S,Lee J,Hansen JK,Boxill MF,Keren B,Marey I,Saenz MS,Brown K,Alexander SA,Mureev S,Batzilla A,Davis MJ,Piper M,Bodén M,Burne THJ,Palpant NJ,Møller RS,Glatt S,Wainwright BJ

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Tue May 11 00:00:00 EDT 2021

Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.

Nature communications

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Tue May 11 00:00:00 EDT 2021

Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.

Journal of experimental & clinical cancer research : CR

Liu C,Pan Z,Chen Q,Chen Z,Liu W,Wu L,Jiang M,Lin W,Zhang Y,Lin W,Zhou R,Zhao L

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Wed Sep 22 00:00:00 EDT 2021

Quantitative analysis of signaling responses during mouse primordial germ cell specification.

Biology open

Morgani SM,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Sat May 15 00:00:00 EDT 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2021

The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos.

eLife

Morgani SM,Su J,Nichols J,Massagué J,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry (Frozen)

Fri Apr 30 00:00:00 EDT 2021

Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.

Frontiers in endocrinology

Peculis R,Mandrika I,Petrovska R,Dortane R,Megnis K,Nazarovs J,Balcere I,Stukens J,Konrade I,Pirags V,Klovins J,Rovite V

53-9811 was used in Immunocytochemistry-immunoflourescence to trace the origin of sphere-forming and adherent pituitary cell cultures and characterize the potential use of these surgery derived cell lines as pituitary adenoma model.

Mon May 10 00:00:00 EDT 2021

Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging.

Frontiers in aging neuroscience

Sugiura A,Shimizu T,Kameyama T,Maruo T,Kedashiro S,Miyata M,Mizutani K,Takai Y

53-9811-80 was used in Immunocytochemistry to identify Sox2 and NeuN double-positive cells in a subpopulation of cells in the mouse arcuate nucleus.

Tue Mar 30 00:00:00 EDT 2021

Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging.

Frontiers in aging neuroscience

Sugiura A,Shimizu T,Kameyama T,Maruo T,Kedashiro S,Miyata M,Mizutani K,Takai Y

53-9811-80 was used in Immunocytochemistry to identify Sox2 and NeuN double-positive cells in a subpopulation of cells in the mouse arcuate nucleus.

Tue Mar 30 00:00:00 EDT 2021

Human induced pluripotent stem cells ameliorate hyperoxia-induced lung injury in a mouse model.

American journal of translational research

Mitchell A,Wanczyk H,Jensen T,Finck C

53-9811 was used in Flow cytometry/Cell sorting to determine if iPSCs and diPSCs could repair hyperoxia-induced lung damage in a mouse model.

Mon Sep 28 00:00:00 EDT 2020

Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction (Cambridge, England)

Ramos-Ibeas P,Lamas-Toranzo I,Martínez-Moro Á,de Frutos C,Quiroga AC,Zurita E,Bermejo-Álvarez P

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.

Viruses

Trus I,Berube N,Jiang P,Rak J,Gerdts V,Karniychuk U

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Mon May 25 00:00:00 EDT 2020

Analysis of Dual Class I Histone Deacetylase and Lysine Demethylase Inhibitor Domatinostat (4SC-202) on Growth and Cellular and Genomic Landscape of Atypical Teratoid/Rhabdoid.

Cancers

Hoffman MM,Zylla JS,Bhattacharya S,Calar K,Hartman TW,Bhardwaj RD,Miskimins WK,de la Puente P,Gnimpieba EZ,Messerli SM

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Mon Mar 23 00:00:00 EDT 2020

Investigating cortical features of Sotos syndrome using mice heterozygous for Nsd1.

Genes, brain, and behavior

Oishi S,Zalucki O,Vega MS,Harkins D,Harvey TJ,Kasherman M,Davila RA,Hale L,White M,Piltz S,Thomas P,Burne THJ,Harris L,Piper M

53-9811-80 was used in Immunocytochemistry to assess the expression of Nsd1 within the mouse brain, and showed a predominantly neuronal pattern of expression for this histone-modifying factor.

Wed Apr 01 00:00:00 EDT 2020

Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System.

Life (Basel, Switzerland)

Siljee S,Pilkington T,Brasch HD,Bockett N,Patel J,Paterson E,Davis PF,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry (Paraffin)

Mon Nov 02 00:00:00 EST 2020

Bcl2-Expressing Quiescent Type B Neural Stem Cells in the Ventricular-Subventricular Zone Are Resistant to Concurrent Temozolomide/X-Irradiation.

Stem cells (Dayton, Ohio)

Cameron BD,Traver G,Roland JT,Brockman AA,Dean D,Johnson L,Boyd K,Ihrie RA,Freeman ML

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Sun Dec 01 00:00:00 EST 2019

FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation.

Cell reports

Lee K,Cho H,Rickert RW,Li QV,Pulecio J,Leslie CS,Huangfu D

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Tue Jul 09 00:00:00 EDT 2019

Glutamine independence is a selectable feature of pluripotent stem cells.

Nature metabolism

Vardhana SA,Arnold PK,Rosen BP,Chen Y,Carey BW,Huangfu D,Carmona Fontaine C,Thompson CB,Finley LWS

53-9811-82 was used in Flow Cytometry to show that reduced dependence on exogenous glutamine is a generalizable feature of pluripotent stem cells.

Mon Jul 01 00:00:00 EDT 2019

Spatial-Temporal Lineage Restrictions of Embryonic p63⁺ Progenitors Establish Distinct Stem Cell Pools in Adult Airways.

Developmental cell

Yang Y,Riccio P,Schotsaert M,Mori M,Lu J,Lee DK,García-Sastre A,Xu J,Cardoso WV

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunohistochemistry

Mon Mar 26 00:00:00 EDT 2018

A morphology independent approach for identifying dividing adult neural stem cells in the mouse hippocampus.

Developmental dynamics : an official publication of the American Association of Anatomists

Harris L,Zalucki O,Oishi S,Burne TH,Jhaveri DJ,Piper M

53-9811 was used in Immunohistochemistry-immunofluorescence to verify using Hes5::GFP mice, that labeling for the cell cycle marker Ki67 and selection against the intermediate progenitor cell marker TBR2 (Ki67+ve ; TBR2-ve nuclei) is sufficient to identify dividing horizontally and radially oriented adult hippocampal neural stem cells in the adult mouse hippocampus.

Mon Jan 01 00:00:00 EST 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.

Nature communications

Lazarus KA,Hadi F,Zambon E,Bach K,Santolla MF,Watson JK,Correia LL,Das M,Ugur R,Pensa S,Becker L,Campos LS,Ladds G,Liu P,Evan GI,McCaughan FM,Le Quesne J,Lee JH,Calado D,Khaled WT

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Mon Aug 20 00:00:00 EDT 2018

Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal Strengths and Limitations of the Teratoma Assay and In Vitro Pluripotency Assays.

Stem cell reports

Bouma MJ,van Iterson M,Janssen B,Mummery CL,Salvatori DCF,Freund C

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunocytochemistry

Tue May 09 00:00:00 EDT 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Flow Cytometry

Wed Mar 15 00:00:00 EDT 2017

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

53981182 was used in immunocytochemistry to investigate if conditioned medium from proliferating fibroblasts induce a subset of hematopoietic cells to become adherent fibroblast-like cells

Wed Jun 01 00:00:00 EDT 2016

Usp9x-deficiency disrupts the morphological development of the postnatal hippocampal dentate gyrus.

Scientific reports

Oishi S,Premarathne S,Harvey TJ,Iyer S,Dixon C,Alexander S,Burne TH,Wood SA,Piper M

53-9811 was used in Immunofluorescence to highlight the critical role of USP9X in the normal morphological development of the postnatal dentate gyrus.

Mon May 16 00:00:00 EDT 2016

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using SOX2 monoclonal antibody (Product # 53-9811-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

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UniProt ID: (Human) P48431, (Mouse) P48432

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Entrez Gene ID: (Human) 6657, (Mouse) 20674

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding transcription factor binding chromatin DNA binding protein heterodimerization activity HMG box transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis

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SOX2 Monoclonal Antibody (Btjce), Alexa Fluor™ 488, eBioscience™

SOX2 Antibody (53-9811-82) in ICC/IF

Product Details

53-9811-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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