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Invitrogen
Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor™ 532, eBioscience™
Product Details
58-4888-80
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Isotype Control
Clone
eBio299Arm
Conjugate
Excitation/Emission Max
534/553 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11220475
Product Specific Information
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells. This should be used at the same concentration as the experimental antibody.
Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG; Immunoglobulin G; ImmunoglobulinG
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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