Invitrogen

iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 532, eBioscience™

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Antibody Testing Data (1)
Published Figures (32)

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iNOS Antibody (58-5920-82) in Flow

BALB/c mouse thioglycolate-elicited peritoneal exudate cells were unstimulated (left) or stimulated overnight with LPS (Product # 00-4976-03) (right). Cells were surface-stained with F4/80 Monoclonal Antibody, APC (Product # 17-4801-82) and then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.06 µg of iNOS Monoclonal Antibody, Alexa Fluor 532. Total viable cells, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18), were used for analysis.

iNOS Antibody in Immunocytochemistry (ICC/IF)

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Product Details

58-5920-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 532, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CXNFT

Conjugate

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

Excitation/Emission Max

534/553 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866448

Product Specific Information

Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.

Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This CXNFT antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Acute murine cytomegalovirus infection boosts cell-type specific response and lipid metabolism changes in the liver of infant mice.

Frontiers in immunology

Gao J,Wang A,Bu X,Jia W

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue Aug 29 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Formaglio P,Alabdullah M,Siokis A,Handschuh J,Sauerland I,Fu Y,Krone A,Gintschel P,Stettin J,Heyde S,Mohr J,Philipsen L,Schröder A,Robert PA,Zhao G,Khailaie S,Dudeck A,Bertrand J,Späth GF,Kahlfuß S,Bousso P,Schraven B,Huehn J,Binder S,Meyer-Hermann M,Müller AJ

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue Dec 14 00:00:00 EST 2021

Prostaglandin E₃ attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Journal of cellular and molecular medicine

Cui J,Shan K,Yang Q,Qi Y,Qu H,Li J,Wang R,Jia L,Chen W,Feng N,Chen YQ

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction.

Experimental and therapeutic medicine

Zhang M,Yang JK,Ma J

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization.

Stem cell research & therapy

Dong B,Wang C,Zhang J,Zhang J,Gu Y,Guo X,Zuo X,Pan H,Hsu AC,Wang G,Wang F

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Immunocytochemistry

Sat May 22 00:00:00 EDT 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation.

Nature communications

Iizasa E,Chuma Y,Uematsu T,Kubota M,Kawaguchi H,Umemura M,Toyonaga K,Kiyohara H,Yano I,Colonna M,Sugita M,Matsuzaki G,Yamasaki S,Yoshida H,Hara H

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair.

Scientific reports

Lo CH,Baratchart E,Basanta D,Lynch CC

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation.

Oxidative medicine and cellular longevity

Lee CB,Seo IH,Chae MW,Park JW,Choi EH,Uhm HS,Baik KY

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Cell reports

Giri J,Das R,Nylen E,Chinnadurai R,Galipeau J

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Innate immune memory through TLR2 and NOD2 contributes to the control of Leptospira interrogans infection.

PLoS pathogens

Santecchia I,Vernel-Pauillac F,Rasid O,Quintin J,Gomes-Solecki M,Boneca IG,Werts C

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models.

Scientific reports

Kang SA,Park MK,Park SK,Choi JH,Lee DI,Song SM,Yu HS

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Thu Apr 25 00:00:00 EDT 2019

Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.

Nature communications

Kurelac I,Iommarini L,Vatrinet R,Amato LB,De Luise M,Leone G,Girolimetti G,Umesh Ganesh N,Bridgeman VL,Ombrato L,Columbaro M,Ragazzi M,Gibellini L,Sollazzo M,Feichtinger RG,Vidali S,Baldassarre M,Foriel S,Vidone M,Cossarizza A,Grifoni D,Kofler B,Malanchi I,Porcelli AM,Gasparre G

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri Feb 22 00:00:00 EST 2019

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression.

Oncotarget

Simonneau M,Frouin E,Huguier V,Jermidi C,Jégou JF,Godet J,Barra A,Paris I,Levillain P,Cordier-Dirikoc S,Pedretti N,Bernard FX,Lecron JC,Morel F,Favot L

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri Nov 23 00:00:00 EST 2018

Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.

Nature communications

Yi S,Zhai J,Niu R,Zhu G,Wang M,Liu J,Huang H,Wang Y,Jing X,Kang L,Song W,Shi Y,Tang H

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Mon Sep 24 00:00:00 EDT 2018

Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency.

Nature cell biology

Giampazolias E,Zunino B,Dhayade S,Bock F,Cloix C,Cao K,Roca A,Lopez J,Ichim G,Proïcs E,Rubio-Patiño C,Fort L,Yatim N,Woodham E,Orozco S,Taraborrelli L,Peltzer N,Lecis D,Machesky L,Walczak H,Albert ML,Milling S,Oberst A,Ricci JE,Ryan KM,Blyth K,Tait SWG

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin-induced mouse model of asthma.

Experimental and therapeutic medicine

Nie H,Wang A,He Q,Yang Q,Liu L,Zhang G,Huang Y,Ding X,Yu H,Hu S

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

TGF-β receptor 1 inhibition prevents stenosis of tissue-engineered vascular grafts by reducing host mononuclear phagocyte activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lee YU,de Dios Ruiz-Rosado J,Mahler N,Best CA,Tara S,Yi T,Shoji T,Sugiura T,Lee AY,Robledo-Avila F,Hibino N,Pober JS,Shinoka T,Partida-Sanchez S,Breuer CK

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection.

Cell reports

Schleicher U,Paduch K,Debus A,Obermeyer S,König T,Kling JC,Ribechini E,Dudziak D,Mougiakakos D,Murray PJ,Ostuni R,Körner H,Bogdan C

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2016

Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine models.

Cancer immunology research

Shen L,Sundstedt A,Ciesielski M,Miles KM,Celander M,Adelaiye R,Orillion A,Ciamporcero E,Ramakrishnan S,Ellis L,Fenstermaker R,Abrams SI,Eriksson H,Leanderson T,Olsson A,Pili R

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

Published figure using iNOS monoclonal antibody (Product # 58-5920-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

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Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a

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UniProt ID: (Mouse) P29477

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Entrez Gene ID: (Mouse) 18126

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Function(s)

actin binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity receptor binding iron ion binding protein binding calmodulin binding beta-catenin binding cAMP-dependent protein kinase regulator activity FMN binding oxidoreductase activity protein kinase binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity cadherin binding metal ion binding flavin adenine dinucleotide binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase

Process(es)

ovulation from ovarian follicle response to hypoxia arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium negative regulation of blood pressure regulation of protein kinase activity positive regulation of vasodilation regulation of insulin secretion positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma

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iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 532, eBioscience™

iNOS Antibody (58-5920-82) in Flow

Product Details

58-5920-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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