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Invitrogen
Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 660, eBioscience™
FIGURE: 1 / 1
Mouse IgG2a kappa Isotype Control (606-4724-81) in Flow
C57BL/6 mouse splenocytes were stained with CD49b (Integrin alpha 2) Monoclonal Antibody, eFluor 450 (Product # 48-5971-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Mouse IgG2a kappa Isotype Control, Alexa Fluor 660 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50). This data was collected on a 5-laser Cytek Aurora full spec... View More
Product Details
606-4724-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBM2a
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896274
Product Specific Information
Description: This is a monoclonal mouse IgG2a kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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