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Invitrogen
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Alexa Fluor™ 660, eBioscience™
FIGURE: 1 / 1
CD274 (PD-L1, B7-H1) Antibody (606-5982-82) in Flow
C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, eFluor 450 (Product # 48-0031-82) and 0.06 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 660 (Product # 606-4321-81) (left) or 0.06 µg of CD274 (PD-L1, B7-H1) Monoclonal Antibody, Alexa Fluor 660 (right). Cells in the lymphocyte gate were used for analysis. This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
Product Details
606-5982-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MIH5
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896298
Product Specific Information
Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1
UniProt ID: (Mouse) Q9EP73
Entrez Gene ID: (Mouse) 60533
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell migration
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
positive regulation of T cell proliferation
negative regulation of T cell proliferation
negative regulation of activated T cell proliferation
toxin transport
negative regulation of tumor necrosis factor superfamily cytokine production
positive regulation of interleukin-10 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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