Invitrogen

IFN gamma Monoclonal Antibody (4S.B3), Alexa Fluor™ 660, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

606-7319-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.015 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4S.B3

Conjugate

Alexa Fluor™ 660 Alexa Fluor™ 660 Alexa Fluor™ 660

Excitation/Emission Max

663/691 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896302

Product Specific Information

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4S.B3 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.

Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)
cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter neutrophil apoptotic process regulation of the force of heart contraction adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response apoptotic process movement of cell or subcellular component humoral immune response cell cycle arrest cell surface receptor signaling pathway positive regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration antigen processing and presentation neutrophil chemotaxis negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response negative regulation of myelination positive regulation of synaptic transmission, cholinergic negative regulation of interleukin-17 production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of tumor necrosis factor production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process positive regulation of T cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat1 protein defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host positive regulation of chemokine biosynthetic process positive regulation of interleukin-12 biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes negative regulation of smooth muscle cell proliferation positive regulation of interleukin-1 beta secretion regulation of insulin secretion T cell receptor signaling pathway sensory perception of mechanical stimulus positive regulation of membrane protein ectodomain proteolysis defense response to virus positive regulation of killing of cells of other organism interferon-gamma-mediated signaling pathway regulation of interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity cellular response to lipopolysaccharide cellular response to interleukin-18 positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway regulation of neuronal action potential positive regulation of exosomal secretion positive regulation of tumor necrosis factor (ligand) superfamily member 11 production regulation of hepatocyte proliferation
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