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Invitrogen
VISTA Monoclonal Antibody (B7H5DS8), PE-eFluor™ 610, eBioscience™
FIGURE: 1 / 1
VISTA Antibody (61-1088-42) in Flow
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, eFluor 450 (Product # 48-0149-42) and Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4714-82) (left) or VISTA Monoclonal Antibody, PE-eFluor 610 (right). Cells in the lymphocyte and monocyte gates were used for analysis.
Product Details
61-1088-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B7H5DS8
Immunogen
VISTA-Fc protein
Conjugate
Excitation/Emission Max
565/606 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848490
Product Specific Information
Description: This B7H5DS8 monoclonal antibody recognizes human VISTA, also known as B7-H5 or PD-1H. This clone has been tested and shown not to cross-react with human B7-H4, B7-H6 and B7-H7. This B7H5DS8 antibody will work in flow cytometry only on live and not paraformaldehyde-fixed cells.
Applications Reported: This B7H5DS8 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B7H5DS8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Death Domain1alpha; PD-H1; PDCD1 homolog; Platelet receptor Gi24; Sisp-1; Stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation
Gene Aliases: B7-H5; B7H5; C10orf54; DD1alpha; GI24; PD-1H; PP2135; SISP1; UNQ730/PRO1412; VISTA; VSIR
UniProt ID: (Human) Q9H7M9
Entrez Gene ID: (Human) 64115
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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