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Invitrogen
CD117 (c-Kit) Monoclonal Antibody (104D2), Super Bright™ 436, eBioscience™
FIGURE: 1 / 3
CD117 (c-Kit) Antibody (62-1178-42) in Flow
Staining of normal human peripheral blood cells with the Human Hematopoietic Lineage Cocktail APC (Product # 22-7776-72), CD34 Monoclonal Antibody, PE (Product # 12-0349-42) and Mouse IgG1 K Isotype Control, Super Bright 436 (Product # 62-4714-82) (left) or CD117 (c-Kit) Monoclonal Antibody, Super Bright 436 (right). Lineage negative/low cells were used for analysis.
Product Details
62-1178-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
104D2
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2734858
Product Specific Information
Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.
Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR
UniProt ID: (Human) P10721
Entrez Gene ID: (Human) 3815
protease binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
stem cell factor receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
cytokine binding
protein homodimerization activity
metal ion binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
transmembrane signal receptor
MAPK cascade
activation of MAPK activity
ovarian follicle development
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
immature B cell differentiation
dendritic cell cytokine production
mast cell chemotaxis
glycosphingolipid metabolic process
inflammatory response
signal transduction
spermatogenesis
spermatid development
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
visual learning
male gonad development
positive regulation of gene expression
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
stem cell population maintenance
lamellipodium assembly
hemopoiesis
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
actin cytoskeleton reorganization
mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
ectopic germ cell programmed cell death
hematopoietic stem cell migration
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of programmed cell death
mast cell degranulation
positive regulation of MAPK cascade
pigmentation
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
protein autophosphorylation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of developmental pigmentation
somatic stem cell division
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of sequence-specific DNA binding transcription factor activity
cell chemotaxis
mast cell differentiation
mast cell proliferation
cellular response to thyroid hormone stimulus
melanocyte migration
melanocyte adhesion
positive regulation of vascular smooth muscle cell differentiation
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