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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), Super Bright™ 436, eBioscience™
FIGURE: 1 / 1
Rat IgG1 kappa Isotype Control (62-4301-82)
Swiss Webster mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and staining buffer (left) or Rat IgG1 kappa Isotype Control, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
62-4301-82
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBRG1
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717005
Product Specific Information
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA.
Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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