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Invitrogen
CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), Super Bright™ 600, eBioscience™
FIGURE: 1 / 10
CD62P (P-Selectin) Antibody (63-0626-82) in Flow
Human platelets were unstimulated (left) or activated by thrombin (right) and stained with CD42b Monoclonal Antibody, PE (Product # 12-0429-42) and Mouse IgG1 kappa Isotype Control, Super Bright 600 (Product # 63-4714-82) (top) or CD62P Monoclonal Antibody, Super Bright 600 (bottom). Total cells were used for analysis.
Product Details
63-0626-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Psel.KO2.3
Conjugate
Excitation/Emission Max
414/601 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802431
Product Specific Information
Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.
Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Psel.KO2.3 antibody has been tested by flow cytometric analysis of thrombin-activated platelets. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)
Gene Aliases: CD62; CD62P; GMP-140; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP
UniProt ID: (Human) P16109, (Mouse) Q01102
Entrez Gene ID: (Human) 6403, (Mouse) 20344
platelet degranulation
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
response to lipopolysaccharide
regulation of integrin activation
calcium-mediated signaling using intracellular calcium source
positive regulation of cell adhesion
defense response to Gram-negative bacterium
leukocyte migration
leukocyte tethering or rolling
single organismal cell-cell adhesion
cellular response to interleukin-6
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