Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 645, eBioscience™

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 78

CD31 (PECAM-1) Antibody (64-0311-82) in Flow

BALB/c mouse bone marrow cells were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, Super Bright 645 (Product # 64-4031-82) (left) or 0.125 µg of CD31 Monoclonal Antibody, Super Bright 645 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993).

CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD31 (PECAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD31 (PECAM-1) Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)

CD31 (PECAM-1) Antibody in Western Blot (WB)

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Product Details

64-0311-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 10 publications 10 publications

Flow Cytometry (Flow)

0.25 µg/test

View 43 publications 43 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

390

Conjugate

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2717091

Product Specific Information

Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 390 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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eLife

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Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A.

Cell reports

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

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Nature communications

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

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Immunity

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

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Fli1⁺ cells transcriptional analysis reveals an Lmo2-Prdm16 axis in angiogenesis.

Proceedings of the National Academy of Sciences of the United States of America

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

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Cellular and molecular life sciences : CMLS

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

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The Journal of cell biology

Schmidt V,Horváth C,Dong H,Blüher M,Qvist P,Wolfrum C,Willnow TE

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Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor.

Frontiers in cell and developmental biology

Cano I,Hu Z,AbuSamra DB,Saint-Geniez M,Ng YSE,Argüeso P,D'Amore PA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Western Blot

Sat Jul 16 00:00:00 EDT 2022

Tendon and motor phenotypes in the mouse model of recessive osteogenesis imperfecta.

eLife

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Dubois-Vedrenne I,Al Delbany D,De Henau O,Robert V,Vernimmen M,Langa F,Lefort A,Libert F,Wittamer V,Parmentier M

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STAR protocols

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Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

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Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

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JCI insight

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Frontiers in immunology

Shen S,Prame Kumar K,Wen SW,Shim R,Wanrooy BJ,Stanley D,Moore RJ,Van TTH,Robert R,Hickey MJ,Wong CHY

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

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Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle.

STAR protocols

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Testosterone metabolites differentially regulate obesogenesis and fat distribution.

Molecular metabolism

Sebo ZL,Rodeheffer MS

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Therapy-Induced Senescence Drives Bone Loss.

Cancer research

Yao Z,Murali B,Ren Q,Luo X,Faget DV,Cole T,Ricci B,Thotala D,Monahan J,van Deursen JM,Baker D,Faccio R,Schwarz JK,Stewart SA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

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Development (Cambridge, England)

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Wed Mar 11 00:00:00 EDT 2020

HSPA12B Secreted by Tumor-Associated Endothelial Cells Might Induce M2 Polarization of Macrophages via Activating PI3K/Akt/mTOR Signaling.

OncoTargets and therapy

Zhou J,Zhang A,Fan L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

Sun Apr 17 00:00:00 EDT 2022

Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury.

eLife

Niethamer TK,Stabler CT,Leach JP,Zepp JA,Morley MP,Babu A,Zhou S,Morrisey EE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Mon Feb 24 00:00:00 EST 2020

Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers.

Nature communications

Okazaki K,Anzawa H,Liu Z,Ota N,Kitamura H,Onodera Y,Alam MM,Matsumaru D,Suzuki T,Katsuoka F,Tadaka S,Motoike I,Watanabe M,Hayasaka K,Sakurada A,Okada Y,Yamamoto M,Suzuki T,Kinoshita K,Sekine H,Motohashi H

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Fri Nov 20 00:00:00 EST 2020

Snail promotes the generation of vascular endothelium by breast cancer cells.

Cell death & disease

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Cell death & disease

Hu HJ,Liang X,Li HL,Du CM,Hao JL,Wang HY,Gu JF,Ni AM,Sun LY,Xiao J,Hu JQ,Yuan H,Dai YS,Jin XT,Zhang KJ,Liu XY

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

Mon Nov 30 00:00:00 EST 2020

Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data.

Genome biology

Patrick R,Humphreys DT,Janbandhu V,Oshlack A,Ho JWK,Harvey RP,Lo KK

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JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

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Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis.

Scientific reports

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

Fri Sep 25 00:00:00 EDT 2020

Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis.

Scientific reports

Ray HC,Corliss BA,Bruce AC,Kesting S,Dey P,Mansour J,Seaman SA,Smolko CM,Mathews C,Dey BK,Owens GK,Peirce SM,Yates PA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

Fri Sep 25 00:00:00 EDT 2020

Nature communications

Mei Y,Han X,Liu Y,Yang J,Sumagin R,Ji P

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

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Exosomes derived from GDNF-modified human adipose mesenchymal stem cells ameliorate peritubular capillary loss in tubulointerstitial fibrosis by activating the SIRT1/eNOS signaling pathway.

Theranostics

Chen L,Wang Y,Li S,Zuo B,Zhang X,Wang F,Sun D

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

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Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy.

EMBO molecular medicine

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Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

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Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy.

EMBO molecular medicine

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Vascular endothelial growth factor pathway promotes osseointegration and CD31^hiEMCN^hi endothelium expansion in a mouse tibial implant model: an animal study.

The bone & joint journal

Ji G,Xu R,Niu Y,Li N,Ivashkiv L,Bostrom MPG,Greenblatt MB,Yang X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

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LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart.

JCI insight

Khalil H,Kanisicak O,Vagnozzi RJ,Johansen AK,Maliken BD,Prasad V,Boyer JG,Brody MJ,Schips T,Kilian KK,Correll RN,Kawasaki K,Nagata K,Molkentin JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Thu Aug 08 00:00:00 EDT 2019

PDGFRα⁺ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland.

Nature communications

Joshi PA,Waterhouse PD,Kasaian K,Fang H,Gulyaeva O,Sul HS,Boutros PC,Khokha R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Mon Apr 15 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Gli1⁺ Pericyte Loss Induces Capillary Rarefaction and Proximal Tubular Injury.

Journal of the American Society of Nephrology : JASN

Kramann R,Wongboonsin J,Chang-Panesso M,Machado FG,Humphreys BD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2017

Obesity reversibly depletes the basal cell population and enhances mammary epithelial cell estrogen receptor alpha expression and progenitor activity.

Breast cancer research : BCR

Chamberlin T,D'Amato JV,Arendt LM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

Wed Nov 29 00:00:00 EST 2017

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice.

Drug delivery

Oyama N,Fuchigami Y,Fumoto S,Sato M,Hagimori M,Shimizu K,Kawakami S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry (Frozen)

Wed Nov 01 00:00:00 EDT 2017

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice.

Drug delivery

Oyama N,Fuchigami Y,Fumoto S,Sato M,Hagimori M,Shimizu K,Kawakami S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry (Frozen)

Wed Nov 01 00:00:00 EDT 2017

β3-Adrenergic Regulation of EPC Features Through Manipulation of the Bone Marrow MSC Niche.

Journal of cellular biochemistry

Vafaei R,Nassiri SM,Siavashi V

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Dynamic Patterns of Clonal Evolution in Tumor Vasculature Underlie Alterations in Lymphocyte-Endothelial Recognition to Foster Tumor Immune Escape.

Cancer research

Corey DM,Rinkevich Y,Weissman IL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2016

Genetic lineage tracing defines myofibroblast origin and function in the injured heart.

Nature communications

Kanisicak O,Khalil H,Ivey MJ,Karch J,Maliken BD,Correll RN,Brody MJ,J Lin SC,Aronow BJ,Tallquist MD,Molkentin JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunocytochemistry

Fri Jul 22 00:00:00 EDT 2016

ADAM17 substrate release in proximal tubule drives kidney fibrosis.

JCI insight

Kefaloyianni E,Muthu ML,Kaeppler J,Sun X,Sabbisetti V,Chalaris A,Rose-John S,Wong E,Sagi I,Waikar SS,Rennke H,Humphreys BD,Bonventre JV,Herrlich A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

Thu Aug 18 00:00:00 EDT 2016

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2015

Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability.

The Journal of biological chemistry

Xu D,Fuster MM,Lawrence R,Esko JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

Fri Jan 07 00:00:00 EST 2011

Resident vascular progenitor cells: an emerging role for non-terminally differentiated vessel-resident cells in vascular biology.

Stem cell research

Kovacic JC,Boehm M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 64-0311-82) in Immunohistochemistry

Thu Jan 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

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Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

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UniProt ID: (Mouse) Q08481

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Entrez Gene ID: (Mouse) 18613

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Function(s)

immunoglobulin receptor superfamily

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CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 645, eBioscience™

CD31 (PECAM-1) Antibody (64-0311-82) in Flow

Product Details

64-0311-82

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Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

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