Proteintech

Bcl-XL Monoclonal Antibody (4C12A6)

Bcl-XL Antibody in Immunocytochemistry (ICC/IF)

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Bcl-XL Antibody (66020-1-IG) in ICC/IF

Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-mouse IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Bcl-XL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Bcl-XL Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

66020-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:20,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:600

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4C12A6

Immunogen

Bcl-XL Fusion Protein Ag18037 (1-233 aa encoded by BC019307)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSQSNRELV VDFLSYKLSQ KGYSWSQFSD VEENRTEAPE GTESEMETPS AINGNPSWHL ADSPAVNGAT GHSSSLDARE VIPMAAVKQA LREAGDEFEL RYRRAFSDLT SQLHITPGTA YQSFEQVVNE LFRDGVNWGR IVAFFSFGGA LCVESVDKEM QVLVSRIAAW MATYLNDHLE PWIQENGGWD TFVELYGNNA AAESRKGQER FNRWFLTGMT VAGVVLLGSL FSRK (1-233 aa encoded by BC019307)

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xian Z,Tian J,Zhao Y,Yi Y,Li C,Han J,Zhang Y,Wang Y,Wang L,Liu S,Pan C,Liu C,Wang D,Meng J,Tang X,Wang F,Liang A

Thu Jun 01 00:00:00 EDT 2023

Depletion of PARP10 inhibits the growth and metastatic potential of oral squamous cell carcinoma.

Frontiers in genetics

Zhou Z,Wei B,Liu Y,Liu T,Zeng S,Gan J,Qi G

Wed Nov 02 00:00:00 EDT 2022

Inhibition of proteasomal deubiquitinases USP14 and UCHL5 overcomes tyrosine kinase inhibitor resistance in chronic myeloid leukaemia.

Clinical and translational medicine

Jiang L,He Q,Chen X,Liu A,Ding W,Zhang H,Chen X,Zhou H,Meng Y,Liu B,Peng G,Wang C,Liu J,Shi X

Thu Sep 01 00:00:00 EDT 2022

Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

Microbiology spectrum

Liu Z,Fu Y,Huang Y,Zeng F,Rao J,Xiao X,Sun X,Jin H,Li J,Yang J,Du W,Liu L

Wed Dec 21 00:00:00 EST 2022

Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

Microbiology spectrum

Liu Z,Fu Y,Huang Y,Zeng F,Rao J,Xiao X,Sun X,Jin H,Li J,Yang J,Du W,Liu L

Wed Dec 21 00:00:00 EST 2022

Mechanism of a new photosensitizer (TBZPy) in the treatment of high-risk human papillomavirus-related diseases.

Photodiagnosis and photodynamic therapy

Li Z,Xiao Z,Feng Y,Wang Q,Teng M

Tue Mar 01 00:00:00 EST 2022

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells.

Journal for immunotherapy of cancer

Zhao S,Wang C,Lu P,Lou Y,Liu H,Wang T,Yang S,Bao Z,Han L,Liang X,Ma C,Gao L

Mon Nov 01 00:00:00 EDT 2021

One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence.

Oxidative medicine and cellular longevity

Qing Y,Li H,Zhao Y,Hu P,Wang X,Yu X,Zhu M,Wang H,Wang Z,Guo Q,Hui H

Mon Jan 31 00:00:00 EST 2022

The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.

Blood cancer journal

Qiao X,Ma J,Knight T,Su Y,Edwards H,Polin L,Li J,Kushner J,Dzinic SH,White K,Wang J,Lin H,Wang Y,Wang L,Wang G,Taub JW,Ge Y

Mon Jun 07 00:00:00 EDT 2021

Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.

Journal of medicinal chemistry

Hao EJ,Li GX,Liang YR,Xie MS,Wang DC,Jiang XH,Cheng JY,Shi ZX,Wang Y,Guo HM

Thu Feb 25 00:00:00 EST 2021

Insights into mechanisms of pranoprofen-induced apoptosis and necroptosis in human corneal stromal cells.

Toxicology letters

Lin Y,Yu M,Fan T

Sun Mar 01 00:00:00 EST 2020

Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis.

Molecular medicine reports

Chen X,Ji S,Si J,Zhang X,Wang X,Guo Y,Zou X

Tue Dec 01 00:00:00 EST 2020

Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-κb pathway.

Biochimica et biophysica acta. Molecular basis of disease

Li YM,Zhang J,Su LJ,Kellum JA,Peng ZY

Fri Mar 01 00:00:00 EST 2019

Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-κb pathway.

Biochimica et biophysica acta. Molecular basis of disease

Li YM,Zhang J,Su LJ,Kellum JA,Peng ZY

Fri Mar 01 00:00:00 EST 2019

Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma.

Journal of cancer research and clinical oncology

Inoue K,Hatano K,Hanamatsu Y,Saigo C,Kito Y,Bunai K,Shibata T,Takeuchi T

Mon Apr 01 00:00:00 EDT 2019

Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma.

Journal of cancer research and clinical oncology

Inoue K,Hatano K,Hanamatsu Y,Saigo C,Kito Y,Bunai K,Shibata T,Takeuchi T

Mon Apr 01 00:00:00 EDT 2019

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Antioxidants & redox signaling

Ren T,Wang J,Zhang H,Yuan P,Zhu J,Wu Y,Huang Q,Guo X,Zhang J,Ji L,Li J,Zhang H,Yang H,Xing J

Fri Apr 20 00:00:00 EDT 2018

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Antioxidants & redox signaling

Ren T,Wang J,Zhang H,Yuan P,Zhu J,Wu Y,Huang Q,Guo X,Zhang J,Ji L,Li J,Zhang H,Yang H,Xing J

Fri Apr 20 00:00:00 EDT 2018

Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo.

Biochemical pharmacology

Su Y,Li X,Ma J,Zhao J,Liu S,Wang G,Edwards H,Taub JW,Lin H,Ge Y

Thu Feb 01 00:00:00 EST 2018

Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo.

Biochemical pharmacology

Su Y,Li X,Ma J,Zhao J,Liu S,Wang G,Edwards H,Taub JW,Lin H,Ge Y

Thu Feb 01 00:00:00 EST 2018

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Autophagy

Huang Q,Zhan L,Cao H,Li J,Lyu Y,Guo X,Zhang J,Ji L,Ren T,An J,Liu B,Nie Y,Xing J

Thu Jun 02 00:00:00 EDT 2016

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Autophagy

Huang Q,Zhan L,Cao H,Li J,Lyu Y,Guo X,Zhang J,Ji L,Ren T,An J,Liu B,Nie Y,Xing J

Thu Jun 02 00:00:00 EDT 2016

A novel regulatory mechanism of Pim-3 kinase stability and its involvement in pancreatic cancer progression.

Molecular cancer research : MCR

Zhang F,Liu B,Wang Z,Yu XJ,Ni QX,Yang WT,Mukaida N,Li YY

Sun Dec 01 00:00:00 EST 2013

A novel regulatory mechanism of Pim-3 kinase stability and its involvement in pancreatic cancer progression.

Molecular cancer research : MCR

Zhang F,Liu B,Wang Z,Yu XJ,Ni QX,Yang WT,Mukaida N,Li YY

Sun Dec 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B-cell leukemia/lymphoma x; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BCLXL; BCLXS; DKFZp781P2092; OTTHUMP00000030550; OTTHUMP00000030552; OTTHUMP00000030555; OTTHUMP00000030556; OTTHUMP00000030557; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3

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Gene Aliases: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; PPP1R52

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UniProt ID: (Human) Q07817, (Mouse) Q64373

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Entrez Gene ID: (Human) 598, (Mouse) 12048

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Function(s)

protein binding protein kinase binding identical protein binding protein homodimerization activity protein heterodimerization activity BH3 domain binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process BH domain binding MDM2/MDM4 family protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Bcl-XL Monoclonal Antibody (4C12A6)

Bcl-XL Antibody (66020-1-IG) in ICC/IF

Product Details

66020-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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