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C9orf72 Monoclonal Antibody (3D2H6)

C9orf72 Antibody in Immunocytochemistry (ICC/IF)

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C9orf72 Antibody (66140-1-IG) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 66140-1-IG (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IGG.

C9orf72 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C9orf72 Antibody in Immunoprecipitation (IP)

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Product Details

66140-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3D2H6

Immunogen

C9orf72 Fusion Protein Ag21080 (1-222 aa encoded by BC020851)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.17 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSTLCPPPS PAVAKTEIAL SGKSPLLAAT FAYWDNILGP RVRHIWAPKT EQVLLSDGEI TFLANHTLNG EILRNAESGA IDVKFFVLSE KGVIIVSLIF DGNWNGDRST YGLSIILPQT ELSFYLPLHR VCVDRLTHII RKGRIWMHKE RQENVQKIIL EGTERMEDQG (1-222 aa encoded by BC020851)

Target Information

C9orf72 may play a role in endosomal trafficking and autophagy. It is known to interact with HNRNPA1, HNRNPA2B1, UBQLN2, and small Rab GTPSases. C9orf72 localizes in the nucleus, cytoplasm, endosome, lysosome, and autophagosome. Mutations in this gene can result in frontotemporal dementia and/or amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo.

Scientific reports

Piao X,Meng D,Zhang X,Song Q,Lv H,Jia Y

Tue Apr 05 00:00:00 EDT 2022

Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats.

Animal models and experimental medicine

Dong W,Zhang L,Sun C,Gao X,Guan F,Li J,Chen W,Ma Y,Zhang L

Tue Sep 01 00:00:00 EDT 2020

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Acta neuropathologica

Saberi S,Stauffer JE,Jiang J,Garcia SD,Taylor AE,Schulte D,Ohkubo T,Schloffman CL,Maldonado M,Baughn M,Rodriguez MJ,Pizzo D,Cleveland D,Ravits J

Thu Mar 01 00:00:00 EST 2018

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Acta neuropathologica

Saberi S,Stauffer JE,Jiang J,Garcia SD,Taylor AE,Schulte D,Ohkubo T,Schloffman CL,Maldonado M,Baughn M,Rodriguez MJ,Pizzo D,Cleveland D,Ravits J

Thu Mar 01 00:00:00 EST 2018

Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Amyotrophic lateral sclerosis & frontotemporal degeneration

Davidson YS,Robinson AC,Rollinson S,Pickering-Brown S,Xiao S,Robertson J,Mann DMA

Thu Feb 01 00:00:00 EST 2018

Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Amyotrophic lateral sclerosis & frontotemporal degeneration

Davidson YS,Robinson AC,Rollinson S,Pickering-Brown S,Xiao S,Robertson J,Mann DMA

Thu Feb 01 00:00:00 EST 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.

Nature neuroscience

Sivadasan R,Hornburg D,Drepper C,Frank N,Jablonka S,Hansel A,Lojewski X,Sterneckert J,Hermann A,Shaw PJ,Ince PG,Mann M,Meissner F,Sendtner M

Thu Dec 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Guanine nucleotide exchange factor C9orf72; Guanine nucleotide exchange factor C9orf72 homolog; protein C9orf72 homolog

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Gene Aliases: AI840585; ALSFTD; C9orf72; DENND9; DENNL72; FTDALS; FTDALS1

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UniProt ID: (Mouse) Q6DFW0, (Human) Q96LT7, (Rat) Q66HC3

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Entrez Gene ID: (Mouse) 73205, (Human) 203228, (Rat) 313155

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Function(s)

protein binding Rab GTPase binding

Process(es)

endocytosis autophagy

Performance Guarantee

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C9orf72 Monoclonal Antibody (3D2H6)

C9orf72 Antibody (66140-1-IG) in ICC/IF

Product Details

66140-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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