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SREBF1 Antibody (66875-1-IG) in ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue).
Product Details
66875-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B6G5
Immunogen
SREBF1 Fusion Protein Ag5484
Conjugate
Form
Liquid
Concentration
1000 µg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Aliquoting is unnecessary for -20°C storage.
Target Information
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
Gene Aliases: BHLHD1; SREBF1; SREBP-1c; SREBP1; SREBP1a
UniProt ID: (Human) P36956
Entrez Gene ID: (Human) 6720
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
protein complex binding
sterol response element binding
protein dimerization activity
negative regulation of transcription from RNA polymerase II promoter
regulation of heart rate by chemical signal
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
aging
circadian rhythm
cholesterol metabolic process
insulin receptor signaling pathway
lipid biosynthetic process
cellular response to starvation
response to glucose
positive regulation of triglyceride biosynthetic process
regulation of fatty acid metabolic process
lung development
positive regulation of histone deacetylation
regulation of protein stability
response to food
response to retinoic acid
response to progesterone
response to glucagon
response to drug
mRNA transcription from RNA polymerase II promoter
fat cell differentiation
positive regulation of cholesterol biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
response to cAMP
cellular response to fatty acid
regulation of mitophagy
regulation of protein targeting to mitochondrion
Performance Guarantee
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