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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™
Product Details
67-4321-82
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBR2a
Conjugate
Excitation/Emission Max
413/702 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2665353
Product Specific Information
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported: APC-eFluor® 780 Rat IgG2a Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgG2a K Isotype Control APC-eFluor® 780 has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody.
Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer ((Product # SB-4400)) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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