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Invitrogen
DNAJC13 Recombinant Rabbit Monoclonal Antibody (11H13L5)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
DNAJC13 Antibody (702773) in ICC/IF
Knockdown of DNAJC13 was achieved by transfecting A-431 cells with specific siRNA (Silencer® select Product # s23548 + s23550). Immunofluorescence analysis was performed on A-431cells (untransfected, panel a-d), transfected with DNAJC13 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-DNAJC13 Recombinant Rabbit Monoclonal Antibody (Product # 702773, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488... View More
Product Details
702773
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
11H13L5
Immunogen
Protein corresponding to human DNAJC13 (aa1301-aa1354) GST Fusion
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716891
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Goat, Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans. It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles. DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 13; DnaJ domain-containing protein RME-8; DnaJ homolog subfamily C member 13; j-domain containing protein, required for endocytosis; receptor mediated endocytosis RME-8; Required for receptor-mediated endocytosis 8; RME-8
Gene Aliases: D030002L11Rik; DNAJC13; Gm1124; KIAA0678; mKIAA0678; PARK21; RME-8; RME8
UniProt ID: (Human) O75165
Entrez Gene ID: (Human) 23317, (Mouse) 235567
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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