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mCherry Antibody (740003THRP) in WB
Western blot was performed using mCherry Recombinant Rat monoclonal antibody (16D7), HRP (Product # 740003THRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: Untransfected, 10 µg (Lane 1), empty vector control, 10 µg (Lane 2), H3-mCherry, 10 µg (Lane 3), H3-mCherry, 5 µg (Lane 4), H3-mCherry, 2 µg (Lane 5), mCherry-GST, 1 µg (Lane 6), mCherry-GST, 0.5 µg (Lane 7), mCherry-GST, 0.25 µg (Lane 8), GST-mCherry, 1 µg (Lane 9), GST-mCherry, 0.5 µg (Lane 10), GST-mCherry, 0.25 µg (Lane 11) were elec... View More
Product Details
740003THRP
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rat
/ IgG2a
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
16D7
Immunogen
full-length protein mCherry
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with proprietary stabilizer
Contains
proprietary preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3099394
Product Specific Information
mCherry recombinant rat monoclonal antibody conjugated with HRP specifically recognizes mCherry at both C and N-terminus of proteins.
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mCherry
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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