Invitrogen

CD25 Monoclonal Antibody (PC61.5), Super Bright™ 780, eBioscience™

37 Published Figures

43 References

View all (229) CD25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (37)

FIGURE: 1 / 38

CD25 Antibody (78-0251-82) in Flow

C57BL/6 mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and 0.06 µg of Rat IgG1 kappa Isotype Control, Super Bright 780 (Product # 78-4301-82) (left) or 0.06 µg of CD25 Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.

CD25 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

78-0251-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 40 publications 40 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, lambda

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PC61.5

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744904

Product Specific Information

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

6-Mercaptopurine potently inhibits recruitment of SHP2 by phosphorylated PD-1 to inhibit PD-1 signalling and enhance T cell function.

Immunology

Liu L,Lei Y,Zheng Z,Zhou X,Chen S,Zeng G,Yu L,Wang P,Chen L

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2023

Regulatory T-cells and IL-5 mediate pain outcomes in a preclinical model of chronic muscle pain.

Molecular pain

Lenert ME,Szabo-Pardi TA,Burton MD

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's disease.

Nature communications

Choi BJ,Park MH,Park KH,Han WH,Yoon HJ,Jung HY,Hong JY,Chowdhury MR,Kim KY,Lee J,Song IS,Pang M,Choi MK,Gulbins E,Reichel M,Kornhuber J,Hong CW,Kim C,Kim SH,Schuchman EH,Jin HK,Bae JS

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

A hydrogen peroxide economizer for on-demand oxygen production-assisted robust sonodynamic immunotherapy.

Theranostics

Jiang Q,Qiao B,Lin X,Cao J,Zhang N,Guo H,Liu W,Zhu L,Xie X,Wan L,Tang R,Liang B,Wang D,Wang Z,Zhou Y,Ran H,Li P

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2022

Glutamine is essential for overcoming the immunosuppressive microenvironment in malignant salivary gland tumors.

Theranostics

Cao S,Hung YW,Wang YC,Chung Y,Qi Y,Ouyang C,Zhong X,Hu W,Coblentz A,Maghami E,Sun Z,Lin HH,Ann DK

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Aug 16 00:00:00 EDT 2022

Neoantigen vaccine-induced CD4 T cells confer protective immunity in a mouse model of multiple myeloma through activation of CD8 T cells against non-vaccine, tumor-associated antigens.

Journal for immunotherapy of cancer

Bekri S,Rodney-Sandy R,Gruenstein D,Mei A,Bogen B,Castle J,Levey D,Cho HJ

78-0251-82 was used in Flow cytometry/Cell sorting to indicate that activation of CD8 CTL against MOPC315-associated antigens not present in the vaccine is one of the major mechanisms of tumor immunity.

Tue Feb 01 00:00:00 EST 2022

Neoantigen cancer vaccine augments anti-CTLA-4 efficacy.

NPJ vaccines

Salvatori E,Lione L,Compagnone M,Pinto E,Conforti A,Ciliberto G,Aurisicchio L,Palombo F

78-0251-82 was used in Flow Cytometry to support the use of NCV delivered by DNA-EP with αCTLA-4 and suggest a new combined therapy for clinical testing.

Wed Feb 02 00:00:00 EST 2022

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Allergen-Specific Treg Cells Upregulated by Lung-Stage S. japonicum Infection Alleviates Allergic Airway Inflammation.

Frontiers in cell and developmental biology

Li Z,Zhang W,Luo F,Li J,Yang W,Zhu B,Wu Q,Wang X,Sun C,Xie Y,Xu B,Wang Z,Qian F,Chen J,Wan Y,Hu W

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Neutralization

Sat Jun 26 00:00:00 EDT 2021

TRPM8 channel augments T-cell activation and proliferation.

Cell biology international

Acharya TK,Tiwari A,Majhi RK,Goswami C

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Positive Effects of Oral Antibiotic Administration in Murine Chronic Graft-Versus-Host Disease.

International journal of molecular sciences

Sato S,Shimizu E,He J,Ogawa M,Asai K,Yazu H,Rusch R,Yamane M,Yang F,Fukuda S,Kawakami Y,Tsubota K,Ogawa Y

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Immunohistochemistry

Sat Apr 03 00:00:00 EDT 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Aug 30 00:00:00 EDT 2021

The effect of fingolimod on regulatory T cells in a mouse model of brain ischaemia.

Journal of neuroinflammation

Malone K,Diaz Diaz AC,Shearer JA,Moore AC,Waeber C

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Sat Jan 30 00:00:00 EST 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis.

BMC endocrine disorders

Wei Y,Jing J,Peng Z,Liu X,Wang X

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.

Cell

Ho JSY,Angel M,Ma Y,Sloan E,Wang G,Martinez-Romero C,Alenquer M,Roudko V,Chung L,Zheng S,Chang M,Fstkchyan Y,Clohisey S,Dinan AM,Gibbs J,Gifford R,Shen R,Gu Q,Irigoyen N,Campisi L,Huang C,Zhao N,Jones JD,van Knippenberg I,Zhu Z,Moshkina N,Meyer L,Noel J,Peralta Z,Rezelj V,Kaake R,Rosenberg B,Wang B,Wei J,Paessler S,Wise HM,Johnson J,Vannini A,Amorim MJ,Baillie JK,Miraldi ER,Benner C,Brierley I,Digard P,Łuksza M,Firth AE,Krogan N,Greenbaum BD,MacLeod MK,van Bakel H,Garcìa-Sastre A,Yewdell JW,Hutchinson E,Marazzi I

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Oct 07 00:00:00 EDT 2020

Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.

The Journal of cell biology

Romero-Wolf M,Shin B,Zhou W,Koizumi M,Rothenberg EV,Hosokawa H

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon Oct 05 00:00:00 EDT 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer.

Nature communications

Buqué A,Bloy N,Perez-Lanzón M,Iribarren K,Humeau J,Pol JG,Levesque S,Mondragon L,Yamazaki T,Sato A,Aranda F,Durand S,Boissonnas A,Fucikova J,Senovilla L,Enot D,Hensler M,Kremer M,Stoll G,Hu Y,Massa C,Formenti SC,Seliger B,Elemento O,Spisek R,André F,Zitvogel L,Delaloge S,Kroemer G,Galluzzi L

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development.

Cell reports

Ramstead AG,Wallace JA,Lee SH,Bauer KM,Tang WW,Ekiz HA,Lane TE,Cluntun AA,Bettini ML,Round JL,Rutter J,O'Connell RM

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Western Blot

Tue Mar 03 00:00:00 EST 2020

PTPN2 Deficiency Enhances Programmed T Cell Expansion and Survival Capacity of Activated T Cells.

Cell reports

Flosbach M,Oberle SG,Scherer S,Zecha J,von Hoesslin M,Wiede F,Chennupati V,Cullen JG,List M,Pauling JK,Baumbach J,Kuster B,Tiganis T,Zehn D

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models.

Anti-cancer drugs

Zhou Y,Fu C,Kong Y,Pan D,Wang Y,Huang S,Li Z,Ning Z,Lu X,Shan S,Xin L

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Sex Differences in Mouse Popliteal Lymph Nodes.

Scientific reports

Dill-Garlow R,Chen KE,Walker AM

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Jan 30 00:00:00 EST 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.

Scientific reports

Uehara M,Li X,Sheikhi A,Zandi N,Walker B,Saleh B,Banouni N,Jiang L,Ordikhani F,Dai L,Yonar M,Vohra I,Kasinath V,Orgill DP,Khademhosseini A,Annabi N,Abdi R

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Thu Apr 25 00:00:00 EDT 2019

Hippo Kinases Mst1 and Mst2 Sense and Amplify IL-2R-STAT5 Signaling in Regulatory T Cells to Establish Stable Regulatory Activity.

Immunity

Shi H,Liu C,Tan H,Li Y,Nguyen TM,Dhungana Y,Guy C,Vogel P,Neale G,Rankin S,Feng Y,Peng J,Tao W,Chi H

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance.

Autophagy

Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Fri Mar 22 00:00:00 EDT 2019

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD25 monoclonal antibody (Product # 78-0251-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; p55 chain; sIL 2R; soluble IL 2 receptor

View more View less

Gene Aliases: CD25; Il2r; Il2ra; Ly-43

View more View less

UniProt ID: (Mouse) P01590

View more View less

Entrez Gene ID: (Mouse) 16184

View more View less

Function(s)

interleukin-2 receptor activity protein binding drug binding interleukin-2 binding transmembrane signal receptor

Process(es)

inflammatory response to antigenic stimulus activation-induced cell death of T cells inflammatory response immune response Notch signaling pathway interleukin-2-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of T cell proliferation T cell homeostasis positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD25 Monoclonal Antibody (PC61.5), Super Bright™ 780, eBioscience™

CD25 Antibody (78-0251-82) in Flow

Product Details

78-0251-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!