GenScript

beta Actin Monoclonal Antibody (2D1D10), HRP, THE™

beta Actin Antibody in Western Blot (WB)

FIGURE: 1 / 1

beta Actin Antibody (A00730-100) in WB

Detection antibody:1 µg/mL THE™ Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) (GenScript, A00730). The signal was developed with LumiSensor TM HRP Substrate Kit (GenScript, L00221500).

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Product Details

A00730-100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

ELISA (ELISA)

0.1-1.0 µg/mL

Miscellaneous PubMed (Misc)

View 17 publications 17 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Fish, Goat, Hamster, Human, Mouse, Pig, Rabbit

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2D1D10

Immunogen

A synthetic peptide DDDIAALVVDNGSG coupled to KLH

Conjugate

HRP HRP HRP

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.

GenScript THE™ beta Actin Antibody [HRP], mAb, Mouse is HRP conjugated THE™ beta Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Negative feedback regulation by HuR controls TRIM21 expression and function in response to UV radiation.

Scientific reports

Guha A,Nag S,Ray PS

Thu Jul 16 00:00:00 EDT 2020

A Novel Role for Necroptosis in the Pathogenesis of Necrotizing Enterocolitis.

Cellular and molecular gastroenterology and hepatology

Werts AD,Fulton WB,Ladd MR,Saad-Eldin A,Chen YX,Kovler ML,Jia H,Banfield EC,Buck RH,Goehring K,Prindle T,Wang S,Zhou Q,Lu P,Yamaguchi Y,Sodhi CP,Hackam DJ

Necrotizing enterocolitis (NEC) is a devastating disease of premature infants characterized by Toll-like receptor 4 (TLR4)-dependent intestinal inflammation and enterocyte death. Given that necroptosis is a proinflammatory cell death process that is linked to bacterial signaling, we investigated its potential role in NEC, and the mechanisms involved.,Human and mouse NEC intestine were analyzed for necroptosis gene expression (ie, RIPK1, RIPK3, and MLKL), and protein activation (phosphorylated RI

Mon May 03 00:00:00 EDT 2021

The mitochondria-targeted antioxidant MitoQ inhibits memory loss, neuropathology, and extends lifespan in aged 3xTg-AD mice.

Molecular and cellular neurosciences

Young ML,Franklin JL

Oxidative stress, likely stemming from dysfunctional mitochondria, occurs before major cognitive deficits and neuropathologies become apparent in Alzheimer's disease (AD) patients and in mouse models of the disease. We previously reported that treating 2- to 7-month-old 3xTg-AD mice with the mitochondria-targeted antioxidant MitoQ (mitoquinone mesylate: [10-(4,5-Dimethoxy-2-methyl-3,6-dioxo-1,4-cyclohexadien-1-yl)decyl](triphenyl)phosphonium methanesulfonate), a period when AD-like pathologies f

Sun Dec 01 00:00:00 EST 2019

Relationship between ETS Transcription Factor ETV1 and TGF-β-regulated SMAD Proteins in Prostate Cancer.

Scientific reports

Oh S,Shin S,Song H,Grande JP,Janknecht R

The ETS transcription factor ETV1 is frequently overexpressed in aggressive prostate cancer which is one underlying cause of this disease. Accordingly transgenic mice that prostate-specifically overexpress ETV1 develop prostatic intraepithelial neoplasia. However progression to the adenocarcinoma stage is stifled in these mice suggesting that inhibitory pathways possibly preclude ETV1 from exerting its full oncogenic potential. Here we provide evidence that TGF-β/SMAD signaling represents s

Mon Jun 03 00:00:00 EDT 2019

Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production.

EBioMedicine

Tong Y,Zhou L,Yang L,Guo P,Cao Y,Qin FX,Liu J

Type I IFN-based therapies against solid malignancies have yielded only limited success. How IFN affects tumor-associated macrophage (TAM) compartment to impact the therapeutic outcomes are not well understood.

Tue Jan 01 00:00:00 EST 2019

Isatin-Schiff base-copper (II) complex induces cell death in p53-positive tumors.

Cell death discovery

Bulatov E,Sayarova R,Mingaleeva R,Miftakhova R,Gomzikova M,Ignatyev Y,Petukhov A,Davidovich P,Rizvanov A,Barlev NA

Medicinal bioinorganic chemistry is a thriving field of drug research for cancer treatment. Transition metal complexes coordinated to essential biological scaffolds represent a highly promising class of compounds for design of novel target-specific therapeutics. We report here the biological evaluation of a novel Isatin-Schiff base derivative and its Cu(II) complex in several tumor cell lines by assessing their effects on cellular metabolism real-time cell proliferation and induction of apoptosi

Wed Oct 04 00:00:00 EDT 2023

Activity of enisamium, an isonicotinic acid derivative, against influenza viruses in differentiated normal human bronchial epithelial cells.

Antiviral chemistry & chemotherapy

Boltz D,Peng X,Muzzio M,Dash P,Thomas PG,Margitich V

New therapeutics for the control of influenza virus infections are needed to alleviate the burden caused by seasonal epidemics and occasional pandemics, and to overcome the potential risk of drug-resistance emergence. Enisamium iodide (Amizon®, Farmak) is currently approved for clinical use for the treatment of influenza in 11 countries which includes Ukraine, Russia, Belarus, Kazakhstan, and Uzbekistan. However, experimental evidence of the antiviral activity of enisamium has not been repor

Mon Jun 10 00:00:00 EDT 2019

Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System.

Journal of virology

Li C,Wang Z,Cao Y,Wang L,Ji J,Chen Z,Deng T,Jiang T,Cheng G,Qin FX

Influenza virus RNA-dependent RNA polymerase consists of three viral protein subunits: PA, PB1 and PB2. Protein-protein interactions (PPIs) of these subunits play pivotal roles in assembling the functional polymerase complex, which is essential for the replication and transcription of influenza virus RNA. Here we developed a highly specific and robust bimolecular luminescence complementation (BiLC) reporter system to facilitate the investigation for influenza virus polymerase complex formation.

Wed Mar 01 00:00:00 EST 2017

Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4⁺ Th Cells.

Journal of immunology (Baltimore, Md. : 1950)

Read KA,Powell MD,Baker CE,Sreekumar BK,Ringel-Scaia VM,Bachus H,Martin RE,Cooley ID,Allen IC,Ballesteros-Tato A,Oestreich KJ

B cell lymphoma-6 (Bcl-6) is a transcriptional repressor that is required for the differentiation of T follicular helper (TFH) cell populations. Currently, the molecular mechanisms underlying the transcriptional regulation of Bcl-6 expression are unclear. In this study, we have identified the Ikaros zinc finger transcription factors Aiolos and Ikaros as novel regulators of Bcl-6. We found that increased expression of Bcl-6 in CD4+ Th cell populations correlated with enhanced enrichment of Aiolos

Sun Oct 01 00:00:00 EDT 2017

NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.

Nature

He Y,Zeng MY,Yang D,Motro B,Núñez G

Inflammasomes are intracellular protein complexes that drive the activation of inflammatory caspases. So far, four inflammasomes involving NLRP1, NLRP3, NLRC4 and AIM2 have been described that recruit the common adaptor protein ASC to activate caspase-1, leading to the secretion of mature IL-1β and IL-18 proteins. The NLRP3 inflammasome has been implicated in the pathogenesis of several acquired inflammatory diseases as well as cryopyrin-associated periodic fever syndromes (CAPS) caused by

Thu Feb 18 00:00:00 EST 2016

Interplay between RNA-binding protein HuR and microRNA-125b regulates p53 mRNA translation in response to genotoxic stress.

RNA biology

Ahuja D,Goyal A,Ray PS

Tumor suppressor protein p53 plays a crucial role in maintaining genomic integrity in response to DNA damage. Regulation of translation of p53 mRNA is a major mode of regulation of p53 expression under genotoxic stress. The AU/U-rich element-binding protein HuR has been shown to bind to p53 mRNA 3'UTR and enhance translation in response to DNA-damaging UVC radiation. On the other hand the microRNA miR-125b is reported to repress p53 expression and stress-induced apoptosis. Here we show that UVC

Tue Nov 01 00:00:00 EDT 2016

IL-7 signalling represses Bcl-6 and the TFH gene program.

Nature communications

McDonald PW,Read KA,Baker CE,Anderson AE,Powell MD,Ballesteros-Tato A,Oestreich KJ

The transcriptional repressor Bcl-6 is linked to the development of both CD4(+) T follicular helper (TFH) and central memory T (TCM) cells. Here, we demonstrate that in response to decreased IL-2 signalling, T helper 1 (TH1) cells upregulate Bcl-6 and co-initiate TFH- and TCM-like gene programs, including expression of the cytokine receptors IL-6Rα and IL-7R. Exposure of this potentially bi-potent cell population to IL-6 favours the TFH gene program, whereas IL-7 signalling represses TFH-a

Fri Jan 08 00:00:00 EST 2016

Trans-presentation of IL-15 modulates STAT5 activation and Bcl-6 expression in TH1 cells.

Scientific reports

Cooley ID,Read KA,Oestreich KJ

During infection naïve CD4(+) T helper cells differentiate into specialized effector subsets based upon environmental signals propagated by the cytokine milieu. Recently this paradigm has been complicated by the demonstration that alterations in the cytokine environment can result in varying degrees of plasticity between effector T helper cell populations. Therefore elucidation of the mechanisms by which cytokines regulate T helper cell differentiation decisions is increasingly important. T

Mon Oct 26 00:00:00 EDT 2015

Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.

Proceedings of the National Academy of Sciences of the United States of America

Overton KW,Spencer SL,Noderer WL,Meyer T,Wang CL

Phenotypic heterogeneity within a population of genetically identical cells is emerging as a common theme in multiple biological systems, including human cell biology and cancer. Using live-cell imaging, flow cytometry, and kinetic modeling, we showed that two states-quiescence and cell cycling-can coexist within an isogenic population of human cells and resulted from low basal expression levels of p21, a Cyclin-dependent kinase (CDK) inhibitor (CKI). We attribute the p21-dependent heterogeneity

Tue Oct 14 00:00:00 EDT 2014

Fertilization-induced autophagy in mouse embryos is independent of mTORC1.

Biology of reproduction

Yamamoto A,Mizushima N,Tsukamoto S

Autophagy is a dynamically regulated intracellular degradation system that is important for cellular processes such as amino acid production during starvation and intracellular quality control. Previously, we reported that autophagy is suppressed in oocytes but is rapidly up-regulated after fertilization. During this period, autophagy is thought to be important for the generation of amino acids from the bulk degradation of maternal proteins that have accumulated during oogenesis. However, the me

Tue Jul 01 00:00:00 EDT 2014

Optimization of oncogene expression through intra-population competition.

Biotechnology journal

Ferreira JP,Wang CL

Although functional roles have been assigned to many genes - e.g., those involved in cell-cycle regulation, growth signaling, or cancer - considerably less is known about the quantitative relationship between their expression level and outcome. We devised an intra-population competition to study oncogene dosage. Cell populations were engineered to express a range of H-Ras oncogene levels. Cells with different levels of H-Ras then "competed" for an increased share of the total cell popu

Sun Dec 01 00:00:00 EST 2013

Oncogenic features of the JMJD2A histone demethylase in breast cancer.

International journal of oncology

Berry WL,Shin S,Lightfoot SA,Janknecht R

Estrogen receptor α (ERα) plays a pivotal role in the genesis of the majority of breast tumors. Consequently, endocrine therapy is now routinely utilized in the clinic for the treatment of ERα-positive breast cancer patients. However, how ERα activity becomes dysregulated in breast cancer cells remains to be elucidated. The aim of this study was to show that the histone demethylase JMJD2A, also known as KDM4A, is capable of forming a complex with ERα in vivo. Moreov

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; cytoskeletal beta actin; gamma non-muscle actin; PS1TP5-binding protein 1

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Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

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UniProt ID: (Rabbit) P29751, (Bovine) P60712, (Human) P60709, (Pig) Q6QAQ1, (Chicken) P60706, (Mouse) P60710, (Chinese hamster) P48975

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Entrez Gene ID: (Rabbit) 100009272, (Bovine) 280979, (Goat) 102179831, (Human) 60, (Pig) 414396, (Chicken) 396526, (Mouse) 11461, (Chinese hamster) 100689477

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Function(s)

protein binding ATP binding kinesin binding Tat protein binding identical protein binding nitric-oxide synthase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton nucleosomal DNA binding protein kinase binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

morphogenesis of a polarized epithelium establishment or maintenance of cell polarity regulation of transmembrane transporter activity negative regulation of protein binding adherens junction assembly apical protein localization synaptic vesicle endocytosis regulation of norepinephrine uptake protein localization to adherens junction cellular response to cytochalasin B postsynaptic actin cytoskeleton organization regulation of protein localization to plasma membrane retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis cell motility

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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beta Actin Monoclonal Antibody (2D1D10), HRP, THE™

beta Actin Antibody (A00730-100) in WB

Product Details

A00730-100

Western Blot (WB)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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