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V5 Tag Antibody (A01735-100) in WB
Western blot analysis of N-terminal 5 tagged fusion protein and C-terminal tagged fusion protein using THE™ 5 Tag Antibody [Biotin], mAb, Mouse (A01735, 1 µg/mL). The signal was developed with Streptaidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor TM HRP Substrate Kit (GenScript, L00221).
Product Details
A01735-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4C12E11
Immunogen
V5 tag peptide conjugated to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 10mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).GenScript THE™ V5 Tag Antibody [Biotin], mAb, Mouse is THE™ V5 Tag Antibody (A01724) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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