Invitrogen

CD45 Monoclonal Antibody (I3/2.3), APC-Cyanine7

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CD45 Antibody (A15395) in Flow

Flow cytometry analysis of CD45 in BALB/c mouse splenocytes. Cells were stained using CD45 Monoclonal Antibody (I3/2.3), APC-Cyanine7 (Product # A15395).

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Product Details

A15395

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Guinea pig, Mouse

Host/Isotype

Rat / IgG2b, lambda

Class

Monoclonal

Type

Antibody

Clone

I3/2.3

Immunogen

Mouse CD45 from T1M1 (Thy-1-c) cells

View immunogen

Conjugate

APC-Cyanine7 APC-Cyanine7 APC-Cyanine7

Excitation/Emission Max

653/782 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.1, with proprietary stabilizer

Contains

<0.1% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534409

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarization.

Nature communications

Zhang FL,Hu Z,Wang YF,Zhang WJ,Zhou BW,Sun QS,Lin ZB,Liu KX

A15395 was used in Flow Cytometry to establish a method for transplanting intestinal organoids into intestinal I/R mice.

Wed Oct 25 00:00:00 EDT 2023

Melatonin regulates microglial polarization to M2 cell via RhoA/ROCK signaling pathway in epilepsy.

Immunity, inflammation and disease

Li P,Ji X,Shan M,Wang Y,Dai X,Yin M,Liu Y,Guan L,Ye L,Cheng H

A15395 was used in Flow cytometry/Cell sorting to find that melatonin had a significant protective effect in LPS-treated BV-2 cells and ROCK-KD cells, while the protective effect of melatonin was significantly attenuated in ROCK-OE cells.

Thu Jun 01 00:00:00 EDT 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using CD45 monoclonal antibody (Product # A15395) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using CD45 monoclonal antibody (Product # A15395) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Myeloid DJ-1 deficiency protects acetaminophen-induced acute liver injury through decreasing inflammatory response.

Aging

Wang B,Li J,Jiao J,Xu M,Luo Y,Wang F,Xia Q,Gao Y,Feng Y,Kong X,Sun X

A15395 was used in Flow Cytometry to suggest DJ-1 as a potential therapeutic and/or prophylactic target of APAP-induced acute liver injury.

Wed Jul 21 00:00:00 EDT 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

A15395 was used in Flow Cytometry to indicate that the gut microbial metabolite PA can attenuate intestinal I/R injury by promoting the release of IL-13 from ILC2s via IL-33/ST2 signaling.

Tue Dec 21 00:00:00 EST 2021

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Nature communications

Choi I,Zhang Y,Seegobin SP,Pruvost M,Wang Q,Purtell K,Zhang B,Yue Z

Published figure using CD45 monoclonal antibody (Product # A15395) in Flow Cytometry

Fri Mar 13 00:00:00 EDT 2020

Synaptic development and neuronal myelination are altered with growth restriction in fetal guinea pigs.

Developmental neuroscience

Piorkowska K,Thompson J,Nygard K,Matushewski B,Hammond R,Richardson B

A15395 was used in Immunohistochemistry to examine aberrant synaptogenesis and myelination of neuronal connections as possible links to neurological sequelae in growth-restricted fetuses.

Mon Jul 06 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (I3/2.3), APC-Cyanine7

CD45 Antibody (A15395) in Flow

Product Details

A15395

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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