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FIGURE: 1 / 1
A4GNT Antibody (PA5-88499) in WB
Western blot analysis of A4GNT in extracts of various cell lines. Samples were incubated with A4GNT Polyclonal antibody (Product # PA5-88499) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88499
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human A4GNT (NP_0572451)
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804956
Product Specific Information
Immunogen sequence: MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK; Positive Samples: SGC-7901, 293T, LO2; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1->4Galbeta->R and is largely associated with the Golgi apparatus membrane.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1,4-N-acetylglucosaminyltransferase; alpha4 GnT; Alpha4GnT; GnTalpha4; MGC149493
Gene Aliases: A4GNT; alpha4GnT
UniProt ID: (Human) Q9UNA3
Entrez Gene ID: (Human) 51146
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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