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ABCA1 Antibody (PA5-22908) in ICC/IF
Immunofluorescent analysis of ABCA1 (green) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a DyLight 488-conjugated ABCA1 polyclonal antibody (Product # PA5-22908) at a dilution of 1:200 for 1 hour at room temperature in 1X PBS containing 1% BSA and 0.3% Triton X-100, and then washed with 1X PBS. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 at 40X magnification. Data cour... View More
Product Details
PA5-22908
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Horse,
Hamster,
Human,
Mustelid,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.66 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152509
Target Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABC 1; ABC 1 antibody; ABC Transporter 1; ABC-1; ABC1 antibody; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ATP-binding cassette transporter A1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP antibody; Cholesterol efflux regulatory protein; membrane-bound; Phospholipid-transporting ATPase ABCA1
Gene Aliases: ABC-1; ABC1; ABCA1; CERP; HDLDT1; TGD
UniProt ID: (Human) O95477, (Mouse) P41233
receptor binding
protein binding
ATP binding
phospholipid binding
phospholipid transporter activity
anion transmembrane transporter activity
cholesterol binding
cholesterol transporter activity
syntaxin binding
small GTPase binding
apolipoprotein binding
apolipoprotein A-I binding
apolipoprotein A-I receptor activity
ATPase activity, coupled to transmembrane movement of substances
ATPase binding
nucleotide binding
transporter activity
ATPase activity
ATP-binding cassette (ABC) transporter
primary active transporter
transporter
peptide secretion
protein lipidation
phagocytosis, engulfment
lysosome organization
G-protein coupled receptor signaling pathway
response to nutrient
cholesterol metabolic process
negative regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol efflux
negative regulation of cholesterol storage
endosomal transport
positive regulation of cAMP biosynthetic process
intracellular cholesterol transport
regulation of Cdc42 protein signal transduction
cholesterol efflux
phospholipid efflux
high-density lipoprotein particle assembly
response to laminar fluid shear stress
apolipoprotein A-I-mediated signaling pathway
lipoprotein metabolic process
response to drug
cholesterol homeostasis
reverse cholesterol transport
phospholipid translocation
interleukin-1 beta secretion
phospholipid homeostasis
response to low-density lipoprotein particle
platelet dense granule organization
cellular response to lipopolysaccharide
cellular response to retinoic acid
cellular response to cholesterol
anion transmembrane transport
transport
phospholipid transport
cholesterol transport
lipoprotein biosynthetic process
transmembrane transport
response to high density lipoprotein particle
ion transmembrane transport
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