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ABCA9 Antibody (PA5-103635) in ICC/IF
Immunocytochemistry analysis of ABCA9 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ABCA9 polyclonal antibody (Product # PA5-103635) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ABCA9(Accession Q8IUA7), corresponding to amino acid residues G1167-L1217.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852969
Product Specific Information
Antibody detects endogenous levels of total ABCA9.
Target Information
ABCA9 (ATP-binding cassette, sub-family A (ABC1), member 9) is a 1,624 amino acid protein belonging to the ABC transporter superfamily and the ABCA family. The ABC1 subfamily is the only major ABC subfamily exclusive to multicellular eukaryotes. Ubiquitously expressed, with high expression in heart, brain and fetal tissues, ABCA9 is a multi-pass membrane protein that contains two ABC transporter domains and exists as five alternatively spliced isoforms. ABCA9 exhibits membrane subcellular localization and may play a role in monocyte differentiation and lipid homeostasis. ABCA9 is up-regulated during monocyte differentiation into macrophages and down-regulated by cholesterol loading of macrophages. Spanning 85 kb, ABCA9 contains 39 exons, with a non-coding first exon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-binding cassette A9; ATP-binding cassette sub-family A member 9; ATP-binding cassette transporter sub-family A member 9; ATP-binding cassette, sub-family A (ABC1), member 9; ATP-binding cassette, subfamily A (ABC1), member 9; DKFZp686F2450; MGC75415
Gene Aliases: ABCA9; EST640918
UniProt ID: (Human) Q8IUA7
Entrez Gene ID: (Human) 10350, (Rat) 287788
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