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ABCB4 Antibody (PA5-100194) in ICC/IF
Immunocytochemistry analysis of ABCB4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ABCB4 polyclonal antibody (Product # PA5-100194) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100194
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ABCB4(Accession P21439), corresponding to amino acid residues C353-Y403.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815724
Product Specific Information
Antibody detects endogenous levels of total MDR3.
Target Information
The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABC20; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; CLCS; GP170; MDR1; MGC163296; Multidrug resistance protein 2; Multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 2; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; P-GP; PGY1; Phosphatidylcholine translocator ABCB4
Gene Aliases: ABC21; ABCB4; GBD1; ICP3; mdr-2; MDR2; MDR2/3; MDR3; PFIC-3; Pgy-2; Pgy2; PGY3
UniProt ID: (Human) P21439, (Mouse) P21440
Entrez Gene ID: (Human) 5244, (Mouse) 18670
protein binding
ATP binding
phospholipid transporter activity
phosphatidylcholine transporter activity
xenobiotic-transporting ATPase activity
ATPase activity, coupled to transmembrane movement of substances
phosphatidylcholine-translocating ATPase activity
nucleotide binding
hydrolase activity
ATPase activity
ATP-binding cassette (ABC) transporter
antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent
positive regulation of antigen processing and presentation of peptide antigen via MHC class I
lipid metabolic process
transport
drug transmembrane transport
positive regulation of cholesterol transport
bile acid secretion
response to drug
xenobiotic transport
phospholipid translocation
transmembrane transport
lipid homeostasis
positive regulation of phospholipid translocation
response to fenofibrate
cellular response to bile acid
positive regulation of phospholipid transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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