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ABCF1 Antibody (PA5-99218) in ICC/IF
Immunofluorescent analysis of ABCF1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ABCF1 polyclonal antibody (Product # PA5-99218) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99218
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ABCF1(Accession Q8NE71), corresponding to amino acid residues R800-E845.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818151
Product Specific Information
Antibody detects endogenous levels of total ABCF1.
Target Information
This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-binding cassette 50; ATP-binding cassette 50 (TNF-alpha stimulated); ATP-binding cassette sub-family F member 1; ATP-binding cassette, sub-family F (GCN20), member 1; TNF-alpha-stimulated ABC protein; TNFalpha-inducible ATP-binding protein
Gene Aliases: ABC27; ABC50; ABCF1; AU041969; D17Wsu166e; GCN20
UniProt ID: (Human) Q8NE71, (Mouse) Q6P542, (Rat) Q6MG08
Entrez Gene ID: (Human) 23, (Mouse) 224742, (Rat) 85493
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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