Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
ACAT1 Antibody (PA5-92402) in WB
Western blot analysis of ACAT1 in various lysates. Samples were incubated with ACAT1 Polyclonal antibody (Product # PA5-92402) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-92402
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_0000101)
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806476
Product Specific Information
Immunogen sequence: MAVLAALLRS GARSRSPLLR RLVQEIRYVE RSYVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQ; Positive Samples: SW620, Raji, NCI-H460, HepG2, U-937, Mouse kidney, Mouse liver, Mouse testis, Mouse small intestine; Cellular Location: Mitochondrion
Target Information
ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-Co A acetyltransferase 1 mitochondrial; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; testicular tissue protein Li 198
Gene Aliases: 6330585C21Rik; ACAT; ACAT1; MAT; RATACAL; T2; THIL
UniProt ID: (Human) P24752, (Rat) P17764, (Mouse) Q8QZT1
Entrez Gene ID: (Human) 38, (Rat) 25014, (Mouse) 110446
acetyl-CoA C-acetyltransferase activity
enzyme binding
protein homodimerization activity
metal ion binding
coenzyme binding
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
acyltransferase
liver development
brain development
branched-chain amino acid catabolic process
response to hormone
response to organic cyclic compound
response to starvation
ketone body biosynthetic process
ketone body catabolic process
protein homooligomerization
adipose tissue development
metanephric proximal convoluted tubule development
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support