Invitrogen

ACE Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 References

View all (41) ACE antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Advanced Verification (1)

FIGURE: 1 / 1

ACE Antibody (PA5-83080)

Relative expression in different tissues in IHC: Detection of differential expression levels of ACE demonstrates antibody specificity. Immunohistochemical analysis of ACE using anti-ACE Polyclonal Antibody (Product #PA5-83080), shows significant staining of ACE in small intestine and shows minimal or weak staining in liver tissues. The relative expression levels of ACE within each tissue is shown using RNA... View More

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ACE Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ACE demonstrates antibody specificity. Immunohistochemical analysis of ACE using anti-ACE Polyclonal Antibody (Product #PA5-83080), shows significant staining of ACE in small intestine and shows minimal or weak staining in liver tissues. The relative expression levels of ACE within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-83080

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:200-1:500

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ACE. Recombinant protein control fragment (Product #RP-101635).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2790236

Product Specific Information

Immunogen sequence: KFVEEYDRTS QVVWNEYAEA NWNYNTNITT ETSKILLQKN MQIANHTLKY GTQARKFDVN QLQNTTIKRI IKKVQDLERA ALPAQELEEY NKILLDMETT YSVATVCHPN GSCLQLEPDL TNVMATSRKY EDLLWAWE

Target Information

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes-the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ACE inhibitors on ACE1, ACE2, and TMPRSS2 expression and spheroid attachment on human endometrial Ishikawa cells.

Reproductive biology

Fernando SR,Chen X,Cheng KW,Wong BP,Qi S,Jiang L,Kodithuwakku SP,Ng EH,Yeung WS,Lee KF

PA5-83080 was used in Western Blotting and Immunohistochemistry to suggest that higher ACE2 expression was found in mid-secretory endometrium and the use of ACE inhibitors did not alter endometrial receptivity for embryo implantation.

Thu Sep 01 00:00:00 EDT 2022

ACE inhibitors on ACE1, ACE2, and TMPRSS2 expression and spheroid attachment on human endometrial Ishikawa cells.

Reproductive biology

Fernando SR,Chen X,Cheng KW,Wong BP,Qi S,Jiang L,Kodithuwakku SP,Ng EH,Yeung WS,Lee KF

Thu Sep 01 00:00:00 EDT 2022

Genetic profiles of familial late-onset Alzheimer's disease in China: The Shanghai FLOAD study.

Genes & diseases

Xie XY,Zhao QH,Huang Q,Dammer E,Chen SD,Ren RJ,Wang G

PA5-83080 was used in Western Blotting to suggest that frame-shift and nonsense mutations in ACE may be involved in LOAD through decreased ACE protein levels without affecting direct processing of APP.

Tue Nov 01 00:00:00 EDT 2022

Colon adenocarcinoma-derived cells possessing stem cell function can be modulated using renin-angiotensin system inhibitors.

PloS one

Munro MJ,Peng L,Wickremesekera SK,Tan ST

PA5-83080 was used in immunohistochemistry (paraffin) to investigate whether cells from colon adenocarcinoma (CA) with a cancer stem cell-like phenotype express renin-angiotensin system (RAS) components, and the effect of RAS inhibitors on CA-derived primary cell lines.

Thu Dec 09 00:00:00 EST 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

PA5-83080 was used in Immunohistochemistry to investigate the expression of components of the renin-angiotensin system (RAS) by cancer stem cell (CSC) subpopulations in metastatic head and neck cutaneous squamous cell carcinoma (mHNcSCC).

Wed Jan 27 00:00:00 EST 2021

Bioinformatics

Protein Aliases: ACE; angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript; Angiotensin-converting enzyme; carboxycathepsin; CD143; CD143 antigen; dipeptidyl carboxypeptidase 1; Dipeptidyl carboxypeptidase I; Kininase II; MGC26566; peptidase P; testicular ECA

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Gene Aliases: ACE; ACE1; CD143; DCP; DCP1; ICH; MVCD3

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UniProt ID: (Human) P12821

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Entrez Gene ID: (Human) 1636

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Function(s)

endopeptidase activity carboxypeptidase activity drug binding metallopeptidase activity exopeptidase activity tripeptidyl-peptidase activity peptidyl-dipeptidase activity zinc ion binding chloride ion binding mitogen-activated protein kinase kinase binding bradykinin receptor binding mitogen-activated protein kinase binding metallodipeptidase activity metalloprotease protease protein modifying enzyme

Process(es)

kidney development blood vessel remodeling angiotensin maturation angiotensin catabolic process in blood regulation of renal output by angiotensin neutrophil mediated immunity antigen processing and presentation of peptide antigen via MHC class I regulation of systemic arterial blood pressure by renin-angiotensin spermatogenesis regulation of blood pressure regulation of smooth muscle cell migration regulation of vasoconstriction mononuclear cell proliferation regulation of vasodilation hormone catabolic process peptide catabolic process beta-amyloid metabolic process arachidonic acid secretion heart contraction regulation of angiotensin metabolic process hematopoietic stem cell differentiation positive regulation of protein tyrosine kinase activity cell proliferation in bone marrow positive regulation of peptidyl-tyrosine autophosphorylation regulation of hematopoietic stem cell proliferation negative regulation of gap junction assembly positive regulation of peptidyl-cysteine S-nitrosylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACE Polyclonal Antibody

ACE Antibody (PA5-83080)

Product Details

PA5-83080

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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