Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
ACOT9 Antibody (PA5-103565) in ICC/IF
Immunocytochemistry analysis of ACOT9 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ACOT9 polyclonal antibody (Product # PA5-103565) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103565
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACOT9(Accession Q9Y305), corresponding to amino acid residues S136-F186.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852901
Product Specific Information
Antibody detects endogenous levels of total ACOT9.
Target Information
Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT9 (acyl-CoA thioesterase 9), also known as ACATE2, MT-ACT48 (mitochondrial acyl-CoA thioesterase of 48 kDa) or CGI-16, is a 406 amino acid member of the acyl-CoA hydrolase protein family. ACOT9 contains a C-terminal 80-amino acid domain that is conserved from mouse to human, suggesting that the C-terminus may confer the catalytic activity of ACOT9. The gene encoding ACOT9 is located on chromosome X and the expressed ACOT9 protein is localized to the mitochondrion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Acyl coenzyme A thioester hydrolase 2; Acyl-CoA thioester hydrolase 9; Acyl-CoA thioesterase 9; acyl-Coenzyme A thioesterase 2, mitochondrial; Acyl-coenzyme A thioesterase 9, mitochondrial; Mitochondrial 48 kDa acyl-CoA thioester hydrolase 1; mitochondrial Acyl-CoA Thioesterase; Mt-ACT48.1; MTE-2; p48; Protein U8
Gene Aliases: 0610041P13Rik; ACATE2; ACOT9; C76421; CGI-16; MT-ACT48; MTACT48; p48; U8
UniProt ID: (Human) Q9Y305, (Mouse) Q9R0X4
Entrez Gene ID: (Human) 23597, (Mouse) 56360
acetyl-CoA hydrolase activity
acyl-CoA hydrolase activity
carboxylic ester hydrolase activity
protein binding
CoA hydrolase activity
palmitoyl-CoA hydrolase activity
hydrolase activity
2-oxoglutaryl-CoA thioesterase activity
2,4,4-trimethyl-3-oxopentanoyl-CoA thioesterase activity
3-isopropylbut-3-enoyl-CoA thioesterase activity
glutaryl-CoA hydrolase activity
esterase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support