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ACSL4 Antibody (MA5-42523) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of FACL4 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with ACSL4 Recombinant Monoclonal Antibody (Product # MA5-42523) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter st... View More
Product Details
MA5-42523
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-33
Immunogen
Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2911664
Product Specific Information
Positive Control: NIH/3T3 cell lysate, HepG2 cell lysate, HUVEC, rat brain tissue, mouse brain tissue, HepG2.
Subcellular Location: Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.
Target Information
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acyl-CoA synthetase 4; Arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 4; fatty acid-Coenzyme A ligase long chain 4; fatty acid-Coenzyme A ligase, long chain 4; fatty-acid-Coenzyme A ligase, long-chain 4; L MRX63; LACS 4; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; Long-chain-fatty-acid--CoA ligase 4; mACS4
Gene Aliases: 9430020A05Rik; ACS4; ACSL4; AU018108; FACL4; LACS4; MRX63; MRX68
UniProt ID: (Human) O60488, (Rat) O35547, (Mouse) Q9QUJ7
Entrez Gene ID: (Human) 2182, (Rat) 113976, (Mouse) 50790
long-chain fatty acid metabolic process
lipid metabolic process
triglyceride metabolic process
response to nutrient
fatty acid transport
long-chain fatty-acyl-CoA biosynthetic process
embryonic process involved in female pregnancy
dendritic spine development
response to interleukin-15
fatty acid metabolic process
lipid biosynthetic process
neuron differentiation
positive regulation of cell growth
negative regulation of prostaglandin secretion
metabolic process
regulation of fatty acid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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