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ACTN2 Polyclonal Antibody

ACTN2 Antibody in Immunocytochemistry (ICC/IF)

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ACTN2 Antibody (14221-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACTN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACTN2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ACTN2 Antibody in Immunoprecipitation (IP)

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Product Details

14221-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACTN2 Fusion Protein Ag5459 (1-301 aa encoded by BC051770)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNQIEPGVQ YNYVYDEDEY MIQEEEWDRD LLLDPAWEKQ QRKTFTAWCN SHLRKAGTQI ENIEEDFRNG LKLMLLLEVI SGERLPKPDR GKMRFHKIAN VNKALDYIAS KGVKLVSIGA EEIVDGNVKM TLGMIWTIIL RFAIQDISVE ETSAKEGLLL WCQRKTAPYR NVNIQNFHTS WKDGLGLCAL IHRHRPDLID YSKLNKDDPI GNINLAMEIA EKHLDIPKML DAEDIVNTPK PDERAIMTYV SCFYHAFAGA EQAETAANRI CKVLAVNQEN ERLMEEYERL ASELLEWIRR TI (1-301 aa encoded by BC051770)

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hitachi K,Kiyofuji Y,Yamaguchi H,Nakatani M,Inui M,Tsuchida K

Sun Jan 01 00:00:00 EST 2023

Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hitachi K,Kiyofuji Y,Yamaguchi H,Nakatani M,Inui M,Tsuchida K

Sun Jan 01 00:00:00 EST 2023

Effects of macrophages on the proliferation and cardiac differentiation of human induced pluripotent stem cells.

Cell communication and signaling : CCS

Long C,Guo R,Han R,Li K,Wan Y,Xu J,Gong X,Zhao Y,Yao X,Liu J

Mon Jul 18 00:00:00 EDT 2022

Identification of differential microRNAs and messenger RNAs resulting from ASXL transcriptional regulator 3 knockdown during during heart development.

Bioengineered

Liu ZQ,Cheng M,Fu F,Li R,Han J,Yang X,Deng Q,Li LS,Lei TY,Li DZ,Liao C

Fri Apr 01 00:00:00 EDT 2022

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Nature communications

Qian W,Hadi T,Silvestro M,Ma X,Rivera CF,Bajpai A,Li R,Zhang Z,Qu H,Tellaoui RS,Corsica A,Zias AL,Garg K,Maldonado T,Ramkhelawon B,Chen W

Wed Jan 26 00:00:00 EST 2022

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Nature communications

Qian W,Hadi T,Silvestro M,Ma X,Rivera CF,Bajpai A,Li R,Zhang Z,Qu H,Tellaoui RS,Corsica A,Zias AL,Garg K,Maldonado T,Ramkhelawon B,Chen W

Wed Jan 26 00:00:00 EST 2022

A catechol bioadhesive for rapid hemostasis and healing of traumatic internal organs and major arteries.

Biomaterials

Wang G,Meng X,Wang P,Wang X,Liu G,Wang DA,Fan C

Thu Dec 01 00:00:00 EST 2022

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Basic research in cardiology

Hulikova A,Park KC,Loonat AA,Gunadasa-Rohling M,Curtis MK,Chung YJ,Wilson A,Carr CA,Trafford AW,Fournier M,Moshnikova A,Andreev OA,Reshetnyak YK,Riley PR,Smart N,Milne TA,Crump NT,Swietach P

Thu Mar 31 00:00:00 EDT 2022

Essential role of MESP1-RING1A complex in cardiac differentiation.

Developmental cell

Liang Q,Wang S,Zhou X,Li Y,Xing S,Sha Y,Yang F,Huang W,Liu N,Li Z,Chen Y,Xu Y,Zhu P,Lan F,Sun N

Mon Nov 21 00:00:00 EST 2022

Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.

Frontiers in cell and developmental biology

Li B,Xiong W,Liang WM,Chiou JS,Lin YJ,Chang ACY

Wed Apr 21 00:00:00 EDT 2021

A ceRNA-associated risk model predicts the poor prognosis for head and neck squamous cell carcinoma patients.

Scientific reports

Xu Y,Xu F,Lv Y,Wang S,Li J,Zhou C,Jiang J,Xie B,He F

Thu Mar 18 00:00:00 EDT 2021

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Frontiers in cell and developmental biology

Gilbert G,Kadur Nagaraju C,Duelen R,Amoni M,Bobin P,Eschenhagen T,Roderick HL,Sampaolesi M,Sipido KR

Tue Nov 23 00:00:00 EST 2021

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Frontiers in cell and developmental biology

Gilbert G,Kadur Nagaraju C,Duelen R,Amoni M,Bobin P,Eschenhagen T,Roderick HL,Sampaolesi M,Sipido KR

Tue Nov 23 00:00:00 EST 2021

Mutation location of HCM-causing troponin T mutations defines the degree of myofilament dysfunction in human cardiomyocytes.

Journal of molecular and cellular cardiology

Schuldt M,Johnston JR,He H,Huurman R,Pei J,Harakalova M,Poggesi C,Michels M,Kuster DWD,Pinto JR,van der Velden J

Fri Jan 01 00:00:00 EST 2021

LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.

Journal of molecular and cellular cardiology

Xu Y,Luo Y,Liang C,Zhang T

Sat Feb 01 00:00:00 EST 2020

LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.

Journal of molecular and cellular cardiology

Xu Y,Luo Y,Liang C,Zhang T

Sat Feb 01 00:00:00 EST 2020

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Progress in biophysics and molecular biology

He X,Li L,Tang M,Zeng Y,Li H,Yu X

Fri Nov 01 00:00:00 EDT 2019

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

Progress in biophysics and molecular biology

He X,Li L,Tang M,Zeng Y,Li H,Yu X

Fri Nov 01 00:00:00 EDT 2019

Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.

Journal of cellular biochemistry

Xu Y,Liang C,Luo Y,Xing W,Zhang T

Sat Jun 01 00:00:00 EDT 2019

MiR-466h-5p induces expression of myocardin with complementary promoter sequences.

Biochemical and biophysical research communications

Luo Y,Liang C,Xu Y,Zhang T

Tue Jun 18 00:00:00 EDT 2019

Early Decline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Doklady. Biochemistry and biophysics

Petrova IO,Tyganov SA,Mirzoev TM,Tsaturyan AK,Kozlovskaya IB,Shenkman BS

Sun Jul 01 00:00:00 EDT 2018

Early Decline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Doklady. Biochemistry and biophysics

Petrova IO,Tyganov SA,Mirzoev TM,Tsaturyan AK,Kozlovskaya IB,Shenkman BS

Sun Jul 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein

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Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23

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UniProt ID: (Human) P35609, (Mouse) Q9JI91

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Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245

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Function(s)

Ras guanyl-nucleotide exchange factor activity integrin binding calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding structural constituent of muscle protein domain specific binding LIM domain binding thyroid hormone receptor coactivator activity titin binding identical protein binding protein homodimerization activity ion channel binding protein dimerization activity actin filament binding FATZ binding titin Z domain binding actin binding ligand-dependent nuclear receptor transcription coactivator activity protein binding, bridging metal ion binding

Process(es)

MAPK cascade platelet degranulation cell adhesion microspike assembly muscle filament sliding regulation of membrane potential regulation of apoptotic process negative regulation of potassium ion transport positive regulation of potassium ion transport positive regulation of GTPase activity sarcomere organization focal adhesion assembly protein homotetramerization actin filament uncapping cardiac muscle cell development phospholipase C-activating angiotensin-activated signaling pathway establishment of protein localization to plasma membrane negative regulation of potassium ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity regulation of nucleic acid-templated transcription negative regulation of protein localization to cell surface positive regulation of receptor activity positive regulation of endocytic recycling positive regulation of cation channel activity muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACTN2 Polyclonal Antibody

ACTN2 Antibody (14221-1-AP) in ICC/IF

Product Details

14221-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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