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ADAM10 Polyclonal Antibody

View all (22) ADAM10 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (7)

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

ADAM10 Antibody (25900-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADAM10 Antibody in Western Blot (WB)

ADAM10 Antibody in Western Blot (WB)

ADAM10 Antibody in Western Blot (WB)

Product Image Gallery

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ADAM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ADAM10 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25900-1-AP (ADAM10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ADAM10 Antibody in Western Blot (WB) — SW 1990 cells were subjected to SDS PAGE followed by western blot with 25900-1-AP (ADAM10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ADAM10 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 25900-1-AP ( ADAM10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

25900-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ADAM10 Fusion Protein Ag23078 (306-512 aa encoded by BC126253)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EQNHDDYCL AYVFTDRDFD DGVLGLAWVG APSGSSGGIC EKSKLYSDGK KKSLNTGIIT VQNYGSHVPP KVSHITFAHE VGHNFGSPHD SGTECTPGES KNLGQKENGN YIMYARATSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGRK CKLKPGKQCS TVCIQVKV (306-512 aa encoded by BC126253)

Target Information

Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome.

Cell reports

Shu X,Wei C,Tu WY,Zhong K,Qi S,Wang A,Bai L,Zhang SX,Luo B,Xu ZZ,Zhang K,Shen C

Tue Feb 28 00:00:00 EST 2023

Amyloid futures in the expanding pathology of brain aging and dementia.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Thorwald MA,Silva J,Head E,Finch CE

Thu Jun 01 00:00:00 EDT 2023

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.

Pharmacological research

Fang X,Guo L,Xing Z,Shi L,Liang H,Li A,Kuang C,Tao B,Yang Q

Tue Mar 01 00:00:00 EST 2022

Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.

Cell proliferation

Huo Y,Yang J,Zheng J,Xu D,Yang M,Tao L,Yao H,Fu X,Yang J,Liu D,Hua R,Zhang J,Sun Y,Hu L,Liu W

Sun May 01 00:00:00 EDT 2022

ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase.

Biochemical and biophysical research communications

Zhang X,Tang L,Zhang Z

Fri Jan 22 00:00:00 EST 2021

ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase.

Biochemical and biophysical research communications

Zhang X,Tang L,Zhang Z

Fri Jan 22 00:00:00 EST 2021

ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase.

Biochemical and biophysical research communications

Zhang X,Tang L,Zhang Z

Fri Jan 22 00:00:00 EST 2021

ADAM10 and ADAM17 are degraded by lysosomal pathway via asparagine endopeptidase.

Biochemical and biophysical research communications

Zhang X,Tang L,Zhang Z

Fri Jan 22 00:00:00 EST 2021

Bottom up proteomics identifies neuronal differentiation pathway networks activated by cathepsin inhibition treatment in neuroblastoma cells that are enhanced by concurrent 13-cis retinoic acid treatment.

Journal of proteomics

Halakos EG,Connell AJ,Glazewski L,Wei S,Mason RW

Wed Feb 10 00:00:00 EST 2021

NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.

Frontiers in cell and developmental biology

Dang NN,Li XB,Zhang M,Han C,Fan XY,Huang SH

Tue Sep 07 00:00:00 EDT 2021

Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer's Disease.

Journal of Alzheimer's disease : JAD

Qian X,Zhang S,Duan L,Yang F,Zhang K,Yan F,Ge S

Wed Sep 15 00:00:00 EDT 2021

The cellular expression and proteolytic processing of the amyloid precursor protein is independent of TDP-43.

Bioscience reports

Hicks DA,Jones AC,Pickering-Brown SM,Hooper NM

Thu Apr 30 00:00:00 EDT 2020

Epigenetic Regulation of a Disintegrin and Metalloproteinase (ADAM) Transcription in Colorectal Cancer Cells: Involvement of β-Catenin, BRG1, and KDM4.

Frontiers in cell and developmental biology

Sun L,Chen B,Wu J,Jiang C,Fan Z,Feng Y,Xu Y

Tue Oct 13 00:00:00 EDT 2020

Spinosin Inhibits Aβ_1-42 Production and Aggregation via Activating Nrf2/HO-1 Pathway.

Biomolecules & therapeutics

Zhang X,Wang J,Gong G,Ma R,Xu F,Yan T,Wu B,Jia Y

Fri May 01 00:00:00 EDT 2020

MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer.

Journal of Cancer

Zhang Z,Xu L,He L,Wang J,Shi X,Li Z,Shi S,Hou K,Teng Y,Qu X

Mon Sep 28 00:00:00 EDT 2020

MiR-891a-5p as a prognostic marker and therapeutic target for hormone receptor-positive breast cancer.

Journal of Cancer

Zhang Z,Xu L,He L,Wang J,Shi X,Li Z,Shi S,Hou K,Teng Y,Qu X

Mon Sep 28 00:00:00 EDT 2020

Liraglutide Ameliorates Hyperhomocysteinemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular Targeting.

Neuroscience bulletin

Zhang Y,Xie JZ,Xu XY,Hu J,Xu T,Jin S,Yang SJ,Wang JZ

Thu Aug 01 00:00:00 EDT 2019

Liraglutide Ameliorates Hyperhomocysteinemia-Induced Alzheimer-Like Pathology and Memory Deficits in Rats via Multi-molecular Targeting.

Neuroscience bulletin

Zhang Y,Xie JZ,Xu XY,Hu J,Xu T,Jin S,Yang SJ,Wang JZ

Thu Aug 01 00:00:00 EDT 2019

Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress.

Life sciences

Li F,Zhang Y,Lu X,Shi J,Gong Q

Tue Oct 01 00:00:00 EDT 2019

Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Chai FN,Ma WY,Zhang J,Xu HS,Li YF,Zhou QD,Li XG,Ye XL

Sun Jul 01 00:00:00 EDT 2018

Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Chai FN,Ma WY,Zhang J,Xu HS,Li YF,Zhou QD,Li XG,Ye XL

Sun Jul 01 00:00:00 EDT 2018

Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Chai FN,Ma WY,Zhang J,Xu HS,Li YF,Zhou QD,Li XG,Ye XL

Sun Jul 01 00:00:00 EDT 2018

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 EDT 2017

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 EDT 2017

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 EDT 2017

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: a disintegrin and metalloprotease domain (ADAM) 10; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase; ADAM; ADAM 10; ADAMs; CD156c; CDw156; Disintegrin and metalloproteinase domain-containing protein 10; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; metalloendopeptidases

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Gene Aliases: 1700031C13Rik; AD10; AD18; ADAM10; CD156c; CDw156; HsT18717; KUZ; kuzbanian; MADM; RAK

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UniProt ID: (Human) O14672, (Mouse) O35598

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Entrez Gene ID: (Human) 102, (Mouse) 11487

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endopeptidase activity metalloendopeptidase activity receptor binding integrin binding protein binding metallopeptidase activity zinc ion binding SH3 domain binding protein kinase binding protein homodimerization activity peptidase activity hydrolase activity SH2 domain binding metal ion binding protease

Process(es)

in utero embryonic development protein phosphorylation membrane protein ectodomain proteolysis negative regulation of cell adhesion Notch signaling pathway Notch receptor processing integrin-mediated signaling pathway cell-cell signaling positive regulation of cell proliferation positive regulation of T cell chemotaxis protein processing extracellular matrix disassembly positive regulation of cell growth positive regulation of cell migration response to tumor necrosis factor monocyte activation ephrin receptor signaling pathway PMA-inducible membrane protein ectodomain proteolysis constitutive protein ectodomain proteolysis proteolysis nucleocytoplasmic transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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