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ADAM12 Antibody (PA5-18314) in ICC/IF
Immunocytochemistry analysis of ADAM12 using ADAM12 Polyclonal Antibody (Product # PA5-18314) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic/plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).
Product Details
PA5-18314
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (AARPLPVSPARALC) corresponding to the N-terminus amino acids of ADAM12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10978276
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
ADAM12 is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a disintegrin and metalloproteinase; ADAM; ADAM 12; ADAMs; Disintegrin and metalloproteinase domain-containing protein 12; Meltrin-alpha; metalloendopeptidases; metalloprotease-disintegrin 12 transmembrane; PRO545; RP11-295J3.5; UNQ346
Gene Aliases: ADAM12; ADAM12-OT1; CAR10; MCMP; MCMPMltna; MLTN; MLTNA; UNQ346/PRO545
UniProt ID: (Human) O43184
Entrez Gene ID: (Human) 8038
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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