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ADAM32 Antibody (PA5-106591) in ICC/IF
Immunocytochemistry analysis of ADAM32 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ADAM32 polyclonal antibody (Product # PA5-106591) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106591
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ADAM32(Accession Q8TC27), corresponding to amino acid residues Q747-N787.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854259
Product Specific Information
Antibody detects endogenous levels of total ADAM32.
Target Information
This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a disintegrin and metalloprotease domain 32; a disintegrin and metalloproteinase; a disintegrin and metalloproteinase domain 32; ADAM; ADAM 32; ADAMs; Disintegrin and metalloproteinase domain-containing protein 32; FLJ26299; FLJ29004; metalloendopeptidases; metalloproteinase 12-like protein; testicular tissue protein Li 13
Gene Aliases: ADAM32; UNQ5982/PRO21340
UniProt ID: (Human) Q8TC27
Entrez Gene ID: (Human) 203102
Performance Guarantee
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