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ADAM8 Antibody (MA5-23870) in WB
Western blot analysis of ADAM8 in recombinant human ADAM8 aa 158-497 . Samples were incubated in ADAM8 monoclonal antibody (Product # MA5-23870) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for ADAM8 aa158-497 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-23870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2B
Class
Monoclonal
Type
Antibody
Clone
143303
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain Asp158-Ser653
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608284
Product Specific Information
In Western blots, less than 5% cross-reactivity with the ectodomains of recombinant human ADAM9, 10, 15, 17, and 19 is observed. This antibody detects an epitope within aa 201-497 of recombinant human ADAM8 which corresponds to the metalloprotease and disintegrin-like domains.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a disintegrin and metalloproteinase; a disintegrin and metalloproteinase domain 8; ADAM; ADAM 8; ADAMs; CD156a; Cell surface antigen MS2; Disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen; metalloendopeptidases; MGC134985
Gene Aliases: ADAM8; CD156; CD156a; MS2
UniProt ID: (Human) P78325
Entrez Gene ID: (Human) 101
cell morphogenesis
angiogenesis
leukocyte migration involved in inflammatory response
positive regulation of acute inflammatory response
proteolysis
inflammatory response
positive regulation of protein processing
single organismal cell-cell adhesion
regulation of cell-cell adhesion
extracellular matrix disassembly
positive regulation of T cell differentiation in thymus
activation of MAPK activity involved in innate immune response
negative regulation of neuron apoptotic process
positive regulation of bone resorption
positive regulation of cell adhesion
lymphocyte chemotaxis
positive regulation of protein secretion
positive regulation of membrane protein ectodomain proteolysis
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
positive regulation of thymocyte apoptotic process
cellular response to hypoxia
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
positive regulation of neutrophil extravasation
positive regulation of fibronectin-dependent thymocyte migration
positive regulation of eosinophil migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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