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ADAP Antibody (PA5-109747) in WB
Western blot analysis of ADAP in extracts of Mouse spleen. Samples were incubated with ADAP Polyclonal antibody (Product # PA5-109747) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-109747
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 678-829 of human FYB (NP_001456.3).
Conjugate
Form
Liquid
Concentration
1.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855158
Product Specific Information
Immunogen sequence: NEGSSFPAPP KQLDMGDEVY DDVDTSDFPV SSAEMSQGTN VGKAKTEEKD LKKLKKQEKE EKDFRKKFKY DGEIRVLYST KVTTSITSKK WGTRDLQVKP GESLEVIQTT DDTKVLCRNE EGKYGYVLRS YLADNDGEIY DDIADGCIYD ND
Target Information
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappa-B family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB-120/130; FYN binding protein FYB-130; FYN-binding protein; FYN-binding protein 1; FYN-T-binding protein; p120/p130; SLAP-130; SLP-76-associated phosphoprotein
Gene Aliases: ADAP; B630013F22Rik; FYB; FYB-120/130; FYB1; p120/130; p120/p130; PRO0823; SLAP-130; SLAP130
UniProt ID: (Human) O15117, (Mouse) O35601
Entrez Gene ID: (Human) 2533, (Mouse) 23880
Performance Guarantee
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