Proteintech

ADARB1 Polyclonal Antibody

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ADARB1 Antibody (22248-1-AP) in WB

Rat brain tissue were subjected to SDS PAGE followed by western blot with 22248-1-AP (ADARB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ADARB1 Antibody in Immunoprecipitation (IP)

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Product Details

22248-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ADARB1 Fusion Protein Ag17743 (176-392 aa encoded by BC065545)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LFNGFETPD KAEPPFYVGS NGDDSFSSSG DLSLSASPVP ASLAQPPLPA LPPFPPPSGK NPVMILNELR PGLKYDFLSE SGESHAKSFV MSVVVDGQFF EGSGRNKKLA KARAAQSALA AIFNLHLDQT PSRQPIPSEG LQLHLPQVLA DAVSRLVLGK FGDLTDNFSS PHARRKVLAG VVMTTGTDVK DAKVISVSTG TKCINGEYMS DRGLALND (176-392 aa encoded by BC065545)

Target Information

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Allelic Expression of RNA Editing Gene ADARB1 in Hepatocellular Carcinoma Treated with Transarterial Chemoembolization.

Pharmacogenomics and personalized medicine

Zeng J,Han L,Wang T,Huang L,Zheng Y,Zhang N,Li Z,Yang M

Tue Mar 28 00:00:00 EDT 2023

Atractylodes lancea volatile oils target ADAR2-miR-181a-5p signaling to mesenchymal stem cell chondrogenic differentiation.

Anatomical record (Hoboken, N.J. : 2007)

Ye S,Si W,Qin W,Yang L,Luo Z,Li Z,Xie Y,Pan H,Li X,Huang Z,Zhu M,Chen D

Fri Dec 01 00:00:00 EST 2023

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Nature

Tang S,Stokasimov E,Cui Y,Pellman D

Wed Jun 01 00:00:00 EDT 2022

Lung fibrosis is induced in ADAR2 overexpressing mice via HuR-induced CTGF signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Soundararajan R,Varanasi SM,Patil SS,Srinivas S,Hernández-Cuervo H,Czachor A,Bulkhi A,Fukumoto J,Galam L,Lockey RF,Kolliputi N

Tue Feb 01 00:00:00 EST 2022

Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma.

Frontiers in pharmacology

Lu C,Chen X,Yan Y,Ren X,Wang X,Peng B,Cai Y,Liang Q,Xu Z,Peng J

Sat Feb 19 00:00:00 EST 2022

Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.

Basic research in cardiology

Kokot KE,Kneuer JM,John D,Rebs S,Möbius-Winkler MN,Erbe S,Müller M,Andritschke M,Gaul S,Sheikh BN,Haas J,Thiele H,Müller OJ,Hille S,Leuschner F,Dimmeler S,Streckfuss-Bömeke K,Meder B,Laufs U,Boeckel JN

Thu Jun 23 00:00:00 EDT 2022

Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.

Basic research in cardiology

Thu Jun 23 00:00:00 EDT 2022

Biological functions of RNA modification patterns that define tumor microenvironment and survival outcomes in testicular germ cell tumors.

American journal of translational research

Tang W,Qian J,Qian S

Wed Oct 19 00:00:00 EDT 2022

A-to-I RNA editing of BLCAP promotes cell proliferation by losing the inhibitory of Rb1 in colorectal cancer.

Experimental cell research

Han F,Hu M,Zhang L,Fan X,Wang J,Lou Z,Wang S,Chen L,Ye Y,Ding Y,Jiao H

Mon Aug 15 00:00:00 EDT 2022

Proline-arginine poly-dipeptide encoded by the C9orf72 repeat expansion inhibits adenosine deaminase acting on RNA.

Journal of neurochemistry

Suzuki H,Matsuoka M

Sun Aug 01 00:00:00 EDT 2021

Downregulated ADARB1 Facilitates Cell Proliferation, Invasion and has Effect on the Immune Regulation in Ovarian Cancer.

Frontiers in bioengineering and biotechnology

Zhu W,Xu Z,Huang M,Wang X,Ren X,Cai Y,Peng B,Liang Q,Chen X,Yan Y

Tue Jan 11 00:00:00 EST 2022

Downregulated ADARB1 Facilitates Cell Proliferation, Invasion and has Effect on the Immune Regulation in Ovarian Cancer.

Frontiers in bioengineering and biotechnology

Zhu W,Xu Z,Huang M,Wang X,Ren X,Cai Y,Peng B,Liang Q,Chen X,Yan Y

Tue Jan 11 00:00:00 EST 2022

Function of low ADARB1 expression in lung adenocarcinoma.

PloS one

Wang X,Xu Z,Ren X,Chen X,Wei J,Lin W,Li Z,Ou C,Gong Z,Yan Y

Wed Mar 11 00:00:00 EDT 2020

Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.

Journal of cellular and molecular medicine

Peng L,Zhang H,Su Y,Shen Q,Du C,Xie H,Li H,Yan J,Shen Z,Jiang W,Xia Y,Xu X,Tang W

Sat Sep 01 00:00:00 EDT 2018

Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.

Journal of cellular and molecular medicine

Peng L,Zhang H,Su Y,Shen Q,Du C,Xie H,Li H,Yan J,Shen Z,Jiang W,Xia Y,Xu X,Tang W

Sat Sep 01 00:00:00 EDT 2018

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Scientific reports

Mirra A,Rossi S,Scaricamazza S,Di Salvio M,Salvatori I,Valle C,Rusmini P,Poletti A,Cestra G,Carrì MT,Cozzolino M

Wed May 17 00:00:00 EDT 2017

Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice.

Scientific reports

Mirra A,Rossi S,Scaricamazza S,Di Salvio M,Salvatori I,Valle C,Rusmini P,Poletti A,Cestra G,Carrì MT,Cozzolino M

Wed May 17 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); Double-stranded RNA-specific editase 1; dsRNA adenosine deaminase; dsRNA adenosine deaminase DRADA2; RED1 homolog; RNA editase; RNA editing deaminase 1; RNA editing deaminase of glutamate receptors; RNA-editing deaminase 1; RNA-editing enzyme 1

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Gene Aliases: ADAR2; ADARB1; DRABA2; DRADA2; RED1

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UniProt ID: (Human) P78563, (Rat) P51400

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Entrez Gene ID: (Human) 104, (Rat) 25367

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Function(s)

double-stranded RNA adenosine deaminase activity mRNA binding protein binding poly(A) RNA binding metal ion binding RNA binding double-stranded RNA binding RNA metabolism protein nucleic acid metabolism protein

Process(es)

adenosine to inosine editing mRNA processing negative regulation of cell proliferation negative regulation of cell migration negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of viral genome replication innate immune response defense response to virus regulation of cell cycle RNA processing mRNA modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ADARB1 Polyclonal Antibody

ADARB1 Antibody (22248-1-AP) in WB

Product Details

22248-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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