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ADARB1 Antibody (PA5-34828) in ICC/IF
ADARB1 Polyclonal Antibody detects ADAR2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ADAR2 protein stained by ADARB1 Polyclonal Antibody (Product # PA5-34828) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-34828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contains a sequence corresponding to a region within amino acids 34 and 273 (P78563) of ADAR2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552180
Product Specific Information
Recommended positive controls: NT2D1.
Predicted reactivity: Human (99%), Mouse (96%), Rat (96%), Chicken (82%), Chimpanzee (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); Double-stranded RNA-specific editase 1; dsRNA adenosine deaminase; dsRNA adenosine deaminase DRADA2; RED1 homolog; RNA editase; RNA editing deaminase 1; RNA-editing deaminase 1; RNA-editing enzyme 1
Gene Aliases: ADAR2; ADARB1; DRABA2; DRADA2; RED1
UniProt ID: (Human) P78563
Entrez Gene ID: (Human) 104
adenosine to inosine editing
mRNA processing
negative regulation of cell proliferation
negative regulation of cell migration
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of viral genome replication
innate immune response
defense response to virus
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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