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ADARB1 Antibody (PA5-36179) in IHC (P)
Immunohistochemistry analysis of ADARB1 in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with ADARB1 polyclonal antibody (Product # PA5-36179) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Product Details
PA5-36179
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 480-530 of human ADAR2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2553405
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 82 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); Double-stranded RNA-specific editase 1; dsRNA adenosine deaminase; dsRNA adenosine deaminase DRADA2; RED1 homolog; RNA editase; RNA editing deaminase 1; RNA editing deaminase of glutamate receptors; RNA-editing deaminase 1; RNA-editing enzyme 1
Gene Aliases: 1700057H01Rik; ADAR2; ADARB1; AW124433; AW558573; BB220382; D10Bwg0447e; DRABA2; DRADA2; RED1
UniProt ID: (Human) P78563, (Rat) P51400, (Mouse) Q91ZS8
Entrez Gene ID: (Human) 104, (Rat) 25367, (Mouse) 110532
adenosine to inosine editing
mRNA processing
negative regulation of cell proliferation
negative regulation of cell migration
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of viral genome replication
innate immune response
defense response to virus
regulation of cell cycle
RNA processing
mRNA modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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