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ADCY2 Antibody (PA5-97247) in WB
Western blot analysis of ADCY2 in extracts of various cell lines. Samples were incubated with ADCY2 Polyclonal antibody (Product # PA5-97247) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97247
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 430-600 of human ADCY2 (NP_0654332)
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2809049
Product Specific Information
Immunogen sequence: VTLEHLNGAY KVEEGDGDIR DPYLKQHLVK TYFVINPKGE RRSPQHLFRP RHTLDGAKMR ASVRMTRYLE SWGAAKPFAH LHHRDSMTTE NGKISTTDVP MGQHNFQNRT LRTKSQKKRF EEELNERMIQ AIDGINAQKQ WLKSEDIQRI SLLFYNKVLE KEYRATALPA F; Positive Samples: SH-SY5Y, Rat eye; Cellular Location: Cell membrane, Cytoplasm, Membrane, Multi-pass membrane protein
Target Information
ADCY2 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate(cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3',5'-cyclic AMP synthetase; AC2; adenylate cyclase 2 (brain); adenylate cyclase II; Adenylate cyclase type 2; Adenylate cyclase type II; Adenylyl cyclase 2; ATP pyrophosphate-lyase 2; type II adenylate cyclase
Gene Aliases: AC2; ADCY2; HBAC2; KIAA1060; mKIAA1060
UniProt ID: (Human) Q08462, (Rat) P26769
Entrez Gene ID: (Human) 108, (Mouse) 210044, (Rat) 81636
magnesium ion binding
adenylate cyclase activity
ATP binding
adenylate cyclase binding
manganese ion binding
protein heterodimerization activity
nucleotide binding
calcium- and calmodulin-responsive adenylate cyclase activity
lyase activity
phosphorus-oxygen lyase activity
G-protein beta/gamma-subunit complex binding
metal ion binding
protein binding
cAMP biosynthetic process
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
cyclic nucleotide biosynthetic process
intracellular signal transduction
renal water homeostasis
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
cAMP-mediated signaling
activation of protein kinase A activity
cellular response to glucagon stimulus
cellular response to forskolin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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