Invitrogen

ADFP Polyclonal Antibody

3 Published Figures

10 References

View all (42) ADFP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (3)

ADFP Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 6

ADFP Antibody (PA1-16972) in IHC (F)

Immunohistochemical analysis of ADFP in mouse liver. Samples were incubated with ADFP polyclonal antibody (Product # PA1-16972) followed by using DAB with hematoxylin counterstain.

ADFP Antibody in Immunocytochemistry (ICC/IF)

ADFP Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ADFP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-16972

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat, Bovine, Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the C-terminal region (within residues 350-425) of mouse ADFP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2223607

Product Specific Information

The target sequence has 88% sequence homology with rat ADFP.

Target Information

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aged lipid-laden microglia display impaired responses to stroke.

EMBO molecular medicine

Arbaizar-Rovirosa M,Pedragosa J,Lozano JJ,Casal C,Pol A,Gallizioli M,Planas AM

Published figure using ADFP polyclonal antibody (Product # PA1-16972) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

Aged lipid-laden microglia display impaired responses to stroke.

EMBO molecular medicine

Arbaizar-Rovirosa M,Pedragosa J,Lozano JJ,Casal C,Pol A,Gallizioli M,Planas AM

Published figure using ADFP polyclonal antibody (Product # PA1-16972) in Immunohistochemistry

Wed Feb 08 00:00:00 EST 2023

Transcriptomics and translatomics identify a robust inflammatory gene signature in brain endothelial cells after ischemic stroke.

Journal of neuroinflammation

Arbaizar-Rovirosa M,Gallizioli M,Lozano JJ,Sidorova J,Pedragosa J,Figuerola S,Chaparro-Cabanillas N,Boya P,Graupera M,Claret M,Urra X,Planas AM

PA1-16972 was used in Immunohistochemistry to investigate gene expression changes in brain endothelial cells after acute ischemic stroke using transcriptomics and translatomics.

Mon Sep 11 00:00:00 EDT 2023

Lipid-loaded tumor-associated macrophages sustain tumor growth and invasiveness in prostate cancer.

The Journal of experimental medicine

Masetti M,Carriero R,Portale F,Marelli G,Morina N,Pandini M,Iovino M,Partini B,Erreni M,Ponzetta A,Magrini E,Colombo P,Elefante G,Colombo FS,den Haan JMM,Peano C,Cibella J,Termanini A,Kunderfranco P,Brummelman J,Chung MWH,Lazzeri M,Hurle R,Casale P,Lugli E,DePinho RA,Mukhopadhyay S,Gordon S,Di Mitri D

PA1-16972 was used in Flow cytometry/Cell sorting to study the correlation between tumor-associated macrophages and prostatic adenocarcinoma in prostate cancer patients.

Mon Feb 07 00:00:00 EST 2022

Lung developmental arrest caused by PDGF-A deletion: consequences for the adult mouse lung.

American journal of physiology. Lung cellular and molecular physiology

Gouveia L,Kraut S,Hadzic S,Vazquéz-Liébanas E,Kojonazarov B,Wu CY,Veith C,He L,Mermelekas G,Schermuly RT,Weissmann N,Betsholtz C,Andrae J

PA1-16972 was used in Immunocytochemistry to indicate that Pdgfa deletion-induced lung developmental arrest has consequences for the adult lung at the morphological and functional level.

Wed Apr 01 00:00:00 EDT 2020

Mex3c mutation affects lactation through impairing milk ejection in female mice.

Bioscience reports

Du Y,Sun D,Li Y

PA1-16972 was used in Immunohistochemistry (Paraffin) to study the effect of the Mex3c mutation on lactation in female mice.

Wed Dec 23 00:00:00 EST 2020

Mex3c mutation affects lactation through impairing milk ejection in female mice.

Bioscience reports

Du Y,Sun D,Li Y

PA1-16972 was used in Immunohistochemistry (Paraffin) to study the effect of the Mex3c mutation on lactation in female mice.

Wed Dec 23 00:00:00 EST 2020

Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets.

Nature communications

Ogasawara Y,Cheng J,Tatematsu T,Uchida M,Murase O,Yoshikawa S,Ohsaki Y,Fujimoto T

Published figure using ADFP polyclonal antibody (Product # PA1-16972) in Immunocytochemistry

Tue Sep 08 00:00:00 EDT 2020

Long-term autophagy is sustained by activation of CCTβ3 on lipid droplets.

Nature communications

Ogasawara Y,Cheng J,Tatematsu T,Uchida M,Murase O,Yoshikawa S,Ohsaki Y,Fujimoto T

Published figure using ADFP polyclonal antibody (Product # PA1-16972) in Immunocytochemistry

Tue Sep 08 00:00:00 EDT 2020

Myonectin deletion promotes adipose fat storage and reduces liver steatosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Little HC,Rodriguez S,Lei X,Tan SY,Stewart AN,Sahagun A,Sarver DC,Wong GW

PA1-16972 was used in Western Blotting to determine myonectin's requirement for metabolic homeostasis in a genetic mouse model.

Mon Jul 01 00:00:00 EDT 2019

PDGF-A signaling is required for secondary alveolar septation and controls epithelial proliferation in the developing lung.

Development (Cambridge, England)

Gouveia L,Betsholtz C,Andrae J

PA1-16972 was used in Immunohistochemistry-immunofluorescence to indicate that PDGF-A signaling is involved in myofibroblast proliferation and migration in the lung.

Tue Apr 10 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Adipophilin; adipose differentiation related protein; Adipose differentiation-related protein; ADRP; ADRP antibody; MGC10598; Perilipin-2

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Gene Aliases: AA407157; ADFP; ADPH; ADRP; PLIN2

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UniProt ID: (Human) Q99541, (Bovine) Q9TUM6, (Mouse) P43883

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Entrez Gene ID: (Human) 123, (Bovine) 280981, (Mouse) 11520, (Rat) 298199

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Function(s)

protein binding molecular_function scaffold/adaptor protein

Process(es)

response to organic cyclic compound long-chain fatty acid transport lipid storage response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ADFP Polyclonal Antibody

ADFP Antibody (PA1-16972) in IHC (F)

Product Details

PA1-16972

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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