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Invitrogen
ADFP Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
ADFP Antibody (PA5-29099) in ICC/IF
Immunofluorescence analysis of ADFP was performed using 70% confluent log phase untreated and H2O2 treated Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ADFP Polyclonal Antibody (Product # PA5-29099) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 4... View More
Product Details
PA5-29099
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 28 and 311 of Human ADFP
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546575
Product Specific Information
Recommended positive controls: H1299, HepG2.
Predicted reactivity: Mouse (82%), Pig (87%), Sheep (86%), Chimpanzee (97%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adipophilin; Adipose differentiation-related protein; ADRP; ADRP antibody; MGC10598; Perilipin-2
Gene Aliases: ADFP; ADRP; PLIN2
UniProt ID: (Human) Q99541
Entrez Gene ID: (Human) 123
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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