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ADH5 Antibody (PA5-94932) in ICC/IF
Immunocytochemistry analysis of ADH5 using anti-ADH5 antibody (Product # PA5-94932) . ADH5 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ADH5 antibody (Product # PA5-94932) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-94932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ADH5 recombinant protein (Position: K212-I374).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806738
Product Specific Information
Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADH-B2; alcohol dehydrogenase (class III), chi polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; class III alcohol dehydrogenase, chi subunit; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; Glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase
Gene Aliases: Adh-2; ADH-3; Adh-5; Adh2; Adh3; ADH5; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p
UniProt ID: (Human) P11766, (Rat) P12711, (Mouse) P28474
Entrez Gene ID: (Human) 128, (Rat) 100145871, (Mouse) 11532
alcohol dehydrogenase (NAD) activity
fatty acid binding
zinc ion binding
electron carrier activity
formaldehyde dehydrogenase activity
protein homodimerization activity
S-(hydroxymethyl)glutathione dehydrogenase activity
C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity
aldo-keto reductase (NADP) activity
isocitrate dehydrogenase activity
mevaldate reductase activity
gluconate dehydrogenase activity
steroid dehydrogenase activity
oxidoreductase activity
epoxide dehydrogenase activity
5-exo-hydroxycamphor dehydrogenase activity
2-hydroxytetrahydrofuran dehydrogenase activity
acetoin dehydrogenase activity
phenylcoumaran benzylic ether reductase activity
D-xylose:NADP reductase activity
L-arabinose:NADP reductase activity
D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
steroid dehydrogenase activity, acting on the CH-CH group of donors
(R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity
3-hydroxymenthone dehydrogenase activity
very long-chain-3-hydroxyacyl-CoA dehydrogenase activity
dihydrotestosterone 17-beta-dehydrogenase activity
(R)-2-hydroxyisocaproate dehydrogenase activity
L-arabinose 1-dehydrogenase (NADP+) activity
L-xylulose reductase (NAD+) activity
metal ion binding
3-ketoglucose-reductase activity
(R)-2-hydroxyglutarate dehydrogenase activity
D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
retinoid metabolic process
respiratory system process
ethanol oxidation
peptidyl-cysteine S-nitrosylation
response to lipopolysaccharide
positive regulation of blood pressure
formaldehyde catabolic process
response to nitrosative stress
response to redox state
ethanol catabolic process
aging
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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