Proteintech

AEG-1/MTDH Polyclonal Antibody

AEG-1/MTDH Antibody in Immunocytochemistry (ICC/IF)

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AEG-1/MTDH Antibody (13860-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

AEG-1/MTDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AEG-1/MTDH Antibody in Western Blot (WB)

AEG-1/MTDH Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13860-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 36 publications 36 publications

Immunohistochemistry (IHC)

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AEG-1/MTDH-Specific Fusion Protein Ag4840 (233-582 aa encoded by BC045642)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Immunogen sequence: EQLTTASFP VGSKKNKGDS HLNVQVSNFK SGKGDSTLQV SSGLNENLTV NGGGWNEKSV KLSSQISAGE EKWNSVSPAS AGKRKAEPSA WSQDTGDANT NGKDWGRSWS DRSIFSGIGS TAEPVSQSTT SDYQWDVSRN QPYIDDEWSG LNGLSSADPN SDWNAPAEEW GNWVDEERAS LLKSQEPIPD DQKVSDDDKE KGEGALPTGK SKKKKKKKKK QGEDNSTAQD TEELEKEIRE DLPVNTSKTR PKQEKAFSLK TISTSDPAEV LVKNSQPIKT LPPATSTEPS VILSKSDSDK SSSQVPPILQ ETDKSKSNTK QNSVPPSQTK SETSWESPKQ IKKKKKARRE T (233-582 aa encoded by BC045642)

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Anoikis patterns exhibit distinct prognostic and immune landscapes in Osteosarcoma.

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Zhang Z,Zhu Z,Fu J,Liu X,Mi Z,Tao H,Fan H

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Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance.

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Tue Sep 13 00:00:00 EDT 2022

Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line.

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Tue Nov 01 00:00:00 EDT 2022

Microparticles from Hyperphosphatemia-Stimulated Endothelial Cells Promote Vascular Calcification Through Astrocyte-Elevated Gene-1.

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Downregulation of Astrocyte Elevated Gene-1 Expression Combined with All-Trans Retinoic Acid Inhibits Development of Vasculogenic Mimicry and Angiogenesis in Glioma.

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Wed Jul 07 00:00:00 EDT 2021

Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas.

Experimental and therapeutic medicine

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Mon Feb 22 00:00:00 EST 2021

AEG-1 silencing attenuates M2-polarization of glioma-associated microglia/macrophages and sensitizes glioma cells to temozolomide.

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Mon Aug 30 00:00:00 EDT 2021

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Scientific reports

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Sat Dec 18 00:00:00 EST 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

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Sat Dec 18 00:00:00 EST 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

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Sat Dec 18 00:00:00 EST 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

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Sat Dec 18 00:00:00 EST 2021

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BMC complementary medicine and therapies

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Thu Mar 01 00:00:00 EST 2018

Metadherin contributes to epithelial-mesenchymal transition and paclitaxel resistance induced by acidic extracellular pH in nasopharyngeal carcinoma.

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Oncotarget

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