Invitrogen

alpha Synuclein Monoclonal Antibody (Syn 211)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

29 References

View all (84) alpha Synuclein antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 30

alpha Synuclein Antibody (AHB0261) in ICC/IF

Immunofluorescence analysis of Synuclein-alpha was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Synuclein-alpha Mouse Monoclonal Antibody (Product # AHB0261) at 1 µg/mL in 1% BSA and incubated for 3 hours at... View More

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Synuclein Antibody in Western Blot (WB)

alpha Synuclein Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

alpha Synuclein Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

alpha Synuclein Antibody in Immunohistochemistry (IHC)

alpha Synuclein Antibody in Immunohistochemistry - Free Floating (IHC (Free))

alpha Synuclein Antibody in ELISA (ELISA)

alpha Synuclein Antibody in Dot Blot (DB)

Product Image Gallery

Product Details

AHB0261

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Avian, Guinea pig, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Syn 211

Immunogen

Recombinant human alpha-synuclein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.2, with 0.1% BSA

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536241

Product Specific Information

A suggested positive control for this product is SHSY-5Y cells. It will not cross-react with mouse or rat.

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Ubiquitin-Specific Protease-13 Improves Behavioral Performance in Alpha-Synuclein Expressing Mice.

International journal of molecular sciences

Liu X,Balaraman K,Lynch CC,Hebron M,Shah PK,Hu S,Stevenson M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Western Blot

Sat Jul 23 00:00:00 EDT 2022

Inhibition of Ubiquitin-Specific Protease-13 Improves Behavioral Performance in Alpha-Synuclein Expressing Mice.

International journal of molecular sciences

Liu X,Balaraman K,Lynch CC,Hebron M,Shah PK,Hu S,Stevenson M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Western Blot

Sat Jul 23 00:00:00 EDT 2022

Vocal changes in a zebra finch model of Parkinson's disease characterized by alpha-synuclein overexpression in the song-dedicated anterior forebrain pathway.

PloS one

Medina CA,Vargas E,Munger SJ,Miller JE

AHB0261 was used in Immunohistochemistry to determine if viral-mediated expression of human wild-type αsyn in the adult song system changes song output.

Fri May 06 00:00:00 EDT 2022

α-Syn overexpression, NRF2 suppression, and enhanced ferroptosis create a vicious cycle of neuronal loss in Parkinson's disease.

Free radical biology & medicine

A A,W C,N N,L M,M D,Zhang DD

AHB0261 was used in Western Blotting to indicate that NRF2 is a critical anti-ferroptotic mediator of neuronal survival, and that the vicious cycle of α-syn overexpression and NRF2 suppression, leading to enhanced neuronal ferroptotic cell death, could represent a targetable and currently untapped means of preventing PD onset and progression.

Tue Nov 01 00:00:00 EDT 2022

Alteration of EIF2 Signaling, Glycolysis, and Dopamine Secretion in Form-Deprived Myopia in Response to 1% Atropine Treatment: Evidence From Interactive iTRAQ-MS and SWATH-MS Proteomics Using a Guinea Pig Model.

Frontiers in pharmacology

Zhu Y,Bian JF,Lu DQ,To CH,Lam CS,Li KK,Yu FJ,Gong BT,Wang Q,Ji XW,Zhang HM,Nian H,Lam TC,Wei RH

AHB0261 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to reveal that retinal EIF2 signaling, glycolysis, and dopamine secretion through SNCA are implicated in atropine treatment of myopia in the FDM-induced guinea pig model.

Wed Feb 16 00:00:00 EST 2022

Aberrant Expression of Circulating MicroRNA Leads to the Dysregulation of Alpha-Synuclein and Other Pathogenic Genes in Parkinson's Disease.

Frontiers in cell and developmental biology

Cai M,Chai S,Xiong T,Wei J,Mao W,Zhu Y,Li X,Wei W,Dai X,Yang B,Liu W,Shu B,Wang M,Lu T,Cai Y,Zheng Z,Mei Z,Zhou Y,Yang J,Zhao J,Shen L,Ho JWK,Chen J,Xiong N

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Western Blot

Fri Sep 10 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Novel Ubiquitin Specific Protease-13 Inhibitors Alleviate Neurodegenerative Pathology.

Metabolites

Liu X,Balaraman K,Lynch CC,Hebron M,Wolf C,Moussa C

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Wed Sep 15 00:00:00 EDT 2021

Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance.

Proceedings of the National Academy of Sciences of the United States of America

Brekk OR,Honey JR,Lee S,Hallett PJ,Isacson O

AHB0261 was used in Immunohistochemistry to determine human PD SN neutral lipid content and distribution in dopaminergic neurons, astrocytes, and microglia relative to age-matched healthy subject controls.

Tue Nov 03 00:00:00 EST 2020

Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance.

Proceedings of the National Academy of Sciences of the United States of America

Brekk OR,Honey JR,Lee S,Hallett PJ,Isacson O

Tue Nov 03 00:00:00 EST 2020

Cell type-specific lipid storage changes in Parkinson's disease patient brains are recapitulated by experimental glycolipid disturbance.

Proceedings of the National Academy of Sciences of the United States of America

Brekk OR,Honey JR,Lee S,Hallett PJ,Isacson O

Tue Nov 03 00:00:00 EST 2020

Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations.

eLife

Cupo RR,Shorter J

AHB0261 was used in Western Blotting to investigate whether Skd3 (encoded by human CLPB) might act as a mitochondrial protein disaggregase in metazoa.

Tue Jun 23 00:00:00 EDT 2020

A protocol for precise comparisons of small vessel disease lesions between ex vivo magnetic resonance imaging and histopathology.

International journal of stroke : official journal of the International Stroke Society

Humphreys CA,Jansen MA,Muñoz Maniega S,González-Castro V,Pernet C,Deary IJ,Al-Shahi Salman R,Wardlaw JM,Smith C

AHB0261 was used in Immunohistochemistry to identify and define the cellular characteristics of small vessel disease lesions in human post-mortem brain tissue, in relation to the lesions seen on neuroimaging.

Mon Apr 01 00:00:00 EDT 2019

Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human α-synuclein.

Acta neuropathologica communications

Krejciova Z,Carlson GA,Giles K,Prusiner SB

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunocytochemistry

Mon May 20 00:00:00 EDT 2019

Amido-bridged nucleic acid (AmNA)-modified antisense oligonucleotides targeting α-synuclein as a novel therapy for Parkinson's disease.

Scientific reports

Uehara T,Choong CJ,Nakamori M,Hayakawa H,Nishiyama K,Kasahara Y,Baba K,Nagata T,Yokota T,Tsuda H,Obika S,Mochizuki H

AHB0261 was used in Immunohistochemistry-immunofluorescence to suggest that AmNA-ASO is a promising therapeutic strategy for SNCA-associated pathology in PD.

Tue May 21 00:00:00 EDT 2019

Bimolecular Fluorescence Complementation of Alpha-synuclein Demonstrates its Oligomerization with Dopaminergic Phenotype in Mice.

EBioMedicine

Cai W,Feng D,Schwarzschild MA,McLean PJ,Chen X

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Thu Mar 01 00:00:00 EST 2018

Bimolecular Fluorescence Complementation of Alpha-synuclein Demonstrates its Oligomerization with Dopaminergic Phenotype in Mice.

EBioMedicine

Cai W,Feng D,Schwarzschild MA,McLean PJ,Chen X

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunohistochemistry

Thu Mar 01 00:00:00 EST 2018

A53T-α-synuclein overexpression in murine locus coeruleus induces Parkinson's disease-like pathology in neurons and glia.

Acta neuropathologica communications

Henrich MT,Geibl FF,Lee B,Chiu WH,Koprich JB,Brotchie JM,Timmermann L,Decher N,Matschke LA,Oertel WH

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunofluorescence

Thu May 10 00:00:00 EDT 2018

DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy.

Frontiers in aging neuroscience

Xu CY,Kang WY,Chen YM,Jiang TF,Zhang J,Zhang LN,Ding JQ,Liu J,Chen SD

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Subthalamic Nucleus Deep Brain Stimulation Does Not Modify the Functional Deficits or Axonopathy Induced by Nigrostriatal α-Synuclein Overexpression.

Scientific reports

Fischer DL,Manfredsson FP,Kemp CJ,Cole-Strauss A,Lipton JW,Duffy MF,Polinski NK,Steece-Collier K,Collier TJ,Gombash SE,Buhlinger DJ,Sortwell CE

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Immunofluorescence

Mon Nov 27 00:00:00 EST 2017

Cortical phosphorylated α-Synuclein levels correlate with brain wave spectra in Parkinson's disease.

Movement disorders : official journal of the Movement Disorder Society

Caviness JN,Lue LF,Hentz JG,Schmitz CT,Adler CH,Shill HA,Sabbagh MN,Beach TG,Walker DG

AHB0261 was used in western blot to analyze the correlation between brain wave spectra in Parkinson's disease and cortical phosphorylated alpha-synuclein levels

Fri Jul 01 00:00:00 EDT 2016

Structural basis for the dissociation of α-synuclein fibrils triggered by pressure perturbation of the hydrophobic core.

Scientific reports

de Oliveira GA,Marques MA,Cruzeiro-Silva C,Cordeiro Y,Schuabb C,Moraes AH,Winter R,Oschkinat H,Foguel D,Freitas MS,Silva JL

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Dot blot

Wed Nov 30 00:00:00 EST 2016

Structural basis for the dissociation of α-synuclein fibrils triggered by pressure perturbation of the hydrophobic core.

Scientific reports

de Oliveira GA,Marques MA,Cruzeiro-Silva C,Cordeiro Y,Schuabb C,Moraes AH,Winter R,Oschkinat H,Foguel D,Freitas MS,Silva JL

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in Dot blot

Wed Nov 30 00:00:00 EST 2016

Salivary total α-synuclein, oligomeric α-synuclein and SNCA variants in Parkinson's disease patients.

Scientific reports

Kang W,Chen W,Yang Q,Zhang L,Zhang L,Wang X,Dong F,Zhao Y,Chen S,Quinn TJ,Zhang J,Chen S,Liu J

AHB0261 was used in western blot to identify oligomeric alpha-synuclein, total alpha-synuclein, and SNCA variants in Parkinson's disease patients

Thu Jun 23 00:00:00 EDT 2016

A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy.

Molecular neurodegeneration

Spencer B,Valera E,Rockenstein E,Trejo-Morales M,Adame A,Masliah E

AHB0261 was used in western blot to reduce alpha-synuclein accumulation in a mouse model of multiple system atrophy by using a brain-targeted, modified neurosin (kallikrein-6)

Wed Sep 23 00:00:00 EDT 2015

Hypoestoxide reduces neuroinflammation and α-synuclein accumulation in a mouse model of Parkinson's disease.

Journal of neuroinflammation

Kim C,Ojo-Amaize E,Spencer B,Rockenstein E,Mante M,Desplats P,Wrasidlo W,Adame A,Nchekwube E,Oyemade O,Okogun J,Chan M,Cottam H,Masliah E

AHB0261 was used in immunohistochemistry to study a mouse model of Parkinson's disease for hypoestoxide to reduce neuroinflammation and alpha-synuclein accumulation

Fri Dec 18 00:00:00 EST 2015

Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.

Acta neuropathologica communications

Bourdenx M,Dovero S,Engeln M,Bido S,Bastide MF,Dutheil N,Vollenweider I,Baud L,Piron C,Grouthier V,Boraud T,Porras G,Li Q,Baekelandt V,Scheller D,Michel A,Fernagut PO,Georges F,Courtine G,Bezard E,Dehay B

AHB0261 was used in immunohistochemistry to study alpha-synuclein overexpression by lack of an additive role of aging in nigrostriatal neurodegeneration

Sat Jul 25 00:00:00 EDT 2015

Using gastrocnemius sEMG and plasma α-synuclein for the prediction of freezing of gait in Parkinson's disease patients.

PloS one

Wang XY,Kang WY,Yang Q,Zhang LY,Chen SD,Liu J

Published figure using alpha Synuclein monoclonal antibody (Product # AHB0261) in ELISA

Wed Oct 29 00:00:00 EDT 2014

Morphological and behavioral impact of AAV2/5-mediated overexpression of human wildtype alpha-synuclein in the rat nigrostriatal system.

PloS one

Gombash SE,Manfredsson FP,Kemp CJ,Kuhn NC,Fleming SM,Egan AE,Grant LM,Ciucci MR,MacKeigan JP,Sortwell CE

AHB0261 was used in immunohistochemistry - free floating to describe the neurodegeneration and functional deficits resulting from injection of recombinant adeno-associated virus-expressing human wildtype α-syn in the rat substantia nigra.

Wed Dec 17 00:00:00 EST 2014

Morphological and behavioral impact of AAV2/5-mediated overexpression of human wildtype alpha-synuclein in the rat nigrostriatal system.

PloS one

Gombash SE,Manfredsson FP,Kemp CJ,Kuhn NC,Fleming SM,Egan AE,Grant LM,Ciucci MR,MacKeigan JP,Sortwell CE

Wed Dec 17 00:00:00 EST 2014

Morphological and behavioral impact of AAV2/5-mediated overexpression of human wildtype alpha-synuclein in the rat nigrostriatal system.

PloS one

Gombash SE,Manfredsson FP,Kemp CJ,Kuhn NC,Fleming SM,Egan AE,Grant LM,Ciucci MR,MacKeigan JP,Sortwell CE

Wed Dec 17 00:00:00 EST 2014

Neuropathology in mice expressing mouse alpha-synuclein.

PloS one

Rieker C,Dev KK,Lehnhoff K,Barbieri S,Ksiazek I,Kauffmann S,Danner S,Schell H,Boden C,Ruegg MA,Kahle PJ,van der Putten H,Shimshek DR

AHB0261 was used in western blot to characterize mice that over express murine alpha-Synuclein

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

View more View less

Gene Aliases: NACP; PARK1; PARK4; PD1; SNCA

View more View less

UniProt ID: (Human) P37840

View more View less

Entrez Gene ID: (Human) 6622

View more View less

Function(s)

magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity membrane trafficking regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

alpha Synuclein Monoclonal Antibody (Syn 211)

alpha Synuclein Antibody (AHB0261) in ICC/IF

Product Details

AHB0261

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!